8,126 results

1. pMY.001

   Plasmid

   #228680

   Purpose

   pTDH3 Promoter for yeast expression with Nterminal tag

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   pTDH3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfb13239

   Plasmid

   #219912

   Purpose

   The base plasmid of TUNEYALI for TF49

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49
   

   Expression

   Yeast

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tol2-TLCV2

   Plasmid

   #196331

   Purpose

   the TLCV2 plasmid with Tol2 recombination sites for integration

   Depositor

   Itamar Harel

   Article

   Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

   Insert

   Cas9-2A-eGFP
   

   Use

   CRISPR; Fish expression

   Expression

   Bacterial, Mammalian, and Y…

   Promoter

   Tight TRE promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ A-SLC-His+Strep-Opa1 (SB254)

   Plasmid

   #227603

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   Opa1
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 253-960 only

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

   Plasmid

   #227608

   Purpose

   Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   aa 2-83 deleted

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

   Plasmid

   #227609

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-544, 651-757 only [TM deleted]

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPICZ A-SLC-His+MFN2-Strep (SB255)

   Plasmid

   #227604

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

   Plasmid

   #227611

   Purpose

   Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   MBP, 10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-88 only

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

   Plasmid

   #227610

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-400, 706-757 [TM and HB2 deleted]

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPICZ A-SLC25A46[∆2-83]-His (SB258)

    Plasmid

    #227607

    Purpose

    Inducible expression of His-tagged SLC25A46 lacking its N-terminal region (∆2-83) in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    aa 2-83 deleted

    Promoter

    AOX1

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFa6-TID-FRB-NLS-3HA-KanMX6

    Plasmid

    #228022

    Purpose

    To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWR58

    Plasmid

    #202795

    Purpose

    integrating bioluminescene reporter for Cas9 activity in S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    P-TDH3/coCBG(first portion)-YUM1
    P-TEF1/ShBle/T-ACT1
    YUM1-coCBG(last portion)/T-ADH2
    URA3 and flanking sequence
    

    Expression

    Yeast

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAM198

    Plasmid

    #227657

    Purpose

    pRS305 His-HRV3C-Rpn5 WT

    Depositor

    Andreas Martin

    Article

    Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

    Insert

    rpn5 (RPN5 Budding Yeast)
    

    Tags

    His-HRV3C-

    Expression

    Yeast

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM200

    Plasmid

    #227659

    Purpose

    pRS305 3XFLAG-HRV3C-Rpn5 WT

    Depositor

    Andreas Martin

    Article

    Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

    Insert

    rpn5 (RPN5 Budding Yeast)
    

    Tags

    3X FLAG-HRV3C-

    Expression

    Yeast

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM201

    Plasmid

    #227660

    Purpose

    pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

    Depositor

    Andreas Martin

    Article

    Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

    Insert

    rpn5 (RPN5 Budding Yeast)
    

    Tags

    3x-FLAG-HRV3C-

    Expression

    Yeast

    Mutation

    (amino acids 121-131) VTENKIF mutated to AAAAAAA

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAM202

    Plasmid

    #227661

    Purpose

    pRS316 Rpn6 promoter-Rpn5 ORF-Rpn5 terminator

    Depositor

    Andreas Martin

    Article

    Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

    Insert

    rpn5 (RPN5 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SJZ6-HIS5

    Plasmid

    #228259

    Purpose

    Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    his5
    

    Expression

    Yeast

    Promoter

    TEF1 S.c.

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TINGL-RR pDRF1

    Plasmid

    #226435

    Purpose

    Expresses TINGL-RR (mTq2-based glucose sensor - O2A - ymScarletI) in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    TINGL-O2A-ymScarletI
    

    Expression

    Yeast

    Promoter

    PMA1

    Available Since

    Dec. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. TINGL pDRF1

    Plasmid

    #226436

    Purpose

    Expresses TINGL (mTq2-based glucose sensor) in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    TINGL
    

    Expression

    Yeast

    Promoter

    PMA1

    Available Since

    Dec. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. TINGL-GR pDRF1

    Plasmid

    #226437

    Purpose

    Expresses TINGL-RR (mTq2-based glucose sensor - O2A - ymNeongreen) in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    TINGL-O2A-ymNeongreen
    

    Expression

    Yeast

    Promoter

    PMA1

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge