7,943 results
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Plasmid#219887PurposeThe base plasmid of TUNEYALI for TF22DepositorInsertContains gRNA targeting TF22 (YALI1_C25288g) and homologous arm matching TF22
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13202
Plasmid#219877PurposeThe base plasmid of TUNEYALI forTF12DepositorInsertContains gRNA targeting TF12 (YALI1_C06870g) and homologous arm matching TF12
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13234
Plasmid#219907PurposeThe base plasmid of TUNEYALI for TF44DepositorInsertContains gRNA targeting TF44 (YALI1_B22561g) and homologous arm matching TF44
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13219
Plasmid#219894PurposeThe base plasmid of TUNEYALI for TF29DepositorInsertContains gRNA targeting TF29 (YALI1_C09133g) and homologous arm matching TF29
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13248
Plasmid#219919PurposeThe base plasmid of TUNEYALI for TF58DepositorInsertContains gRNA targeting TF58 (YALI1_F37742g) and homologous arm matching TF58
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-PGAL-GFP-Cps1
Plasmid#207030PurposeExpression of GFP-Cps1 under GAL promoter.DepositorInsertCps1
UseTagsGFPExpressionYeastMutationPromoterGAL1Available sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7repeats)-GFP-Pho8
Plasmid#207011PurposeTet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ypq2-2GFP-URA
Plasmid#207010PurposeIntegration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertYPQ2
UseTags2xGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Vph1-2GFP-URA
Plasmid#207009PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertVPH1
UseTags2xGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Pho8-406
Plasmid#207006PurposeIntegration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.DepositorInsertPHO8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL475
Plasmid#219503PurposeExpress Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in transDepositorInsertAde2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL452
Plasmid#219502PurposeGolden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4tDepositorInsertGAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL390
Plasmid#219500PurposeIntegrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpressionDepositorInsertIntegrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT
UseTagsSV40-NLSExpressionYeastMutationPromoterGAL1-GAL10Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL391
Plasmid#219501PurposeExpress Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequenceDepositorInsertEco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-100
Plasmid#219408PurposeReporter gene (4 operators) for zinc finger activatorDepositorInsert(42-10 op)x4-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-422
Plasmid#219410PurposeReporter gene (1 operator) for zinc finger activatorDepositorInsert(42-10 op)x1-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-424
Plasmid#219412PurposeReporter gene (3 operator) for zinc finger activatorDepositorInsert(42-10 op)x3-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-095
Plasmid#219407PurposeReporter gene (4 operators) for zinc finger activatorDepositorInsert(13-6 op)x4-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-423
Plasmid#219411PurposeReporter gene (2 operator) for zinc finger activatorDepositorInsert(42-10 op)x2-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL2-138
Plasmid#219413PurposeEstradiol-inducible expression of 42-10 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
UseTagsExpressionYeastMutationFour Arg to Ala mutations in zinc finger backbonePromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only