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pCRISPEY-Z3-Hyg

Plasmid

#232101

Purpose

Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-AVO1

Plasmid

#232889

Purpose

Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

AVO1 gRNA (AVO1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HIS3-3

Plasmid

#232882

Purpose

Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

HIS3 gRNA (HIS3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-YFR054C

Plasmid

#232900

Purpose

Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

YFR054C gRNA (YFR054C Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ZIM17

Plasmid

#232901

Purpose

Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ZIM17 gRNA (ZIM17 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-SRP14

Plasmid

#232896

Purpose

Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

SRP14 gRNA (SRP14 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-TLG1

Plasmid

#232897

Purpose

Plasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

TLG1 gRNA (TLG1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-VHT1

Plasmid

#232898

Purpose

Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

VHT1 gRNA (VHT1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HIS3-2

Plasmid

#232881

Purpose

Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

HIS3 gRNA (HIS3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ADE13

Plasmid

#232887

Purpose

Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ADE13 gRNA (ADE13 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-LAS17

Plasmid

#232892

Purpose

Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LAS17 gRNA (LAS17 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#232883

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-INO80

Plasmid

#232890

Purpose

Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

INO80 gRNA (INO80 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-Z3-Nat

Plasmid

#232094

Purpose

Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

Z3 promoter and Z3EV transcription factor

Use

CRISPR

Expression

Yeast

Promoter

Z3

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-K.l.LEU2

Plasmid

#229229

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Publication

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Publication

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PAF24

Plasmid

#223172

Purpose

Expresses VC83_00191-yeGFP under control of CTR1 promoter and terminator

Depositor

Ryan Peterson

Publication

Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

Insert

Copper Transporter

Tags

StreptagII and yeGFP

Expression

Yeast

Promoter

saccharomyces cerevisiae CTR1

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDRF1 ymScarletI3

Plasmid

#224106

Purpose

Expressing ymScarletI3 in yeast

Depositor

Bas Teusink

Insert

ymScarletI3

Expression

Yeast

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymBaojin-URA3

Plasmid

#224081

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mBaojin with URA3 selection

Depositor

Bas Teusink

Insert

ymBaoJin

Tags

ymBaoJin

Expression

Yeast

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDRF1 ymBaojin

Plasmid

#224085

Purpose

Expressing ymBaojin in yeast

Depositor

Bas Teusink

Insert

ymBaoJin

Expression

Yeast

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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