8,129 results

1. pJE13HD7

   Plasmid

   #218281

   Purpose

   Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFP4QE2Crimson

   Plasmid

   #218280

   Purpose

   Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRHDGAL43C

   Plasmid

   #218279

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRGAL43C

   Plasmid

   #218278

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRGAL4A

   Plasmid

   #218277

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRHDGAL3C

   Plasmid

   #218276

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRGAL3C

   Plasmid

   #218275

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPOM014_P2_pGPM1

   Plasmid

   #216439

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter gpm1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Available Since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRH3128

   Plasmid

   #210165

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ADE1-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::ADE1-GFP::tADH1 (ADE2 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRH2941

    Plasmid

    #210143

    Purpose

    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1

    Depositor

    Randy Hampton

    Article

    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

    Insert

    YIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1 (ARO7 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG426-GAL-Prepro-3x-Beta-Amyloid

    Plasmid

    #181730

    Purpose

    Galactose inducible expression of Prepro-3x-Beta-Amyloid

    Depositor

    Alejandro Chavez

    Insert

    Prepro-3x-Beta-Amyloid
    

    Expression

    Yeast

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG426-GAL-Prepro-3x-Adan

    Plasmid

    #181733

    Purpose

    Galactose inducible expression of Prepro-3x-Adan

    Depositor

    Alejandro Chavez

    Insert

    Prepro-3x-Adan
    

    Expression

    Yeast

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG416-GAL-TDP-43-M337V

    Plasmid

    #181721

    Purpose

    Galactose inducible expression of TDP-43-M337V

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Mutation

    M337V

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG416-GAL-TDP-43-N267S

    Plasmid

    #181720

    Purpose

    Galactose inducible expression of TDP-43-N267S

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Mutation

    N267S

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG416-GAL-TDP-43-Q331K

    Plasmid

    #181719

    Purpose

    Galactose inducible expression of TDP-43-Q331K

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Mutation

    Q331K

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG416-GAL-FUS-R521C

    Plasmid

    #181718

    Purpose

    Galactose inducible expression of FUS-R521C

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Expression

    Yeast

    Mutation

    R521C

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG416-GAL-FUS-P431L

    Plasmid

    #181717

    Purpose

    Galactose inducible expression of FUS-P431L

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Expression

    Yeast

    Mutation

    P431L

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG416-GAL-RNQ1

    Plasmid

    #181706

    Purpose

    Galactose inducible expression of RNQ1

    Depositor

    Alejandro Chavez

    Insert

    RNQ1 (RNQ1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p4A8_M59I_T94M_BC

    Plasmid

    #217833

    Purpose

    4A8 variant in barcoded, yeast surface display plasmid

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    4A8 variant Fab
    

    Expression

    Yeast

    Mutation

    VH: M59I, VL: T94M

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYSD_lambda_mRFP

    Plasmid

    #217831

    Purpose

    Yeast surface display Fab vector with lambda light chain and mRFP

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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