8,388 results

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pPICZ A-SLC25A46-His (SB250)

Plasmid

#227599

Purpose

Inducible expression of His-tagged SLC25A46 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46[R247A,R347A]-His (SB257)

Plasmid

#227606

Purpose

Inducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Mutation

R247A,R347A

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1(MGD) (SB252)

Plasmid

#227601

Purpose

Inducible expression of Strep-tagged Opa1(MGD) in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-580 (MGD) and aa 938-960

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13247

Plasmid

#219918

Purpose

The base plasmid of TUNEYALI for TF57

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13228

Plasmid

#219903

Purpose

The base plasmid of TUNEYALI for TF38

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13210

Plasmid

#219885

Purpose

The base plasmid of TUNEYALI for TF20

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

Plasmid

#227084

Purpose

Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus

Depositor

Chris Hill

Insert

spt6-tsh2 (SPT6 Budding Yeast)

Tags

STREPii-FLAG and SV40 NLS 1

Expression

Yeast

Promoter

Gal1-10

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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CUP1-∆ssCPY*-mScarletI

Plasmid

#221157

Purpose

Fluorescent reporter for cytoplasmic aggregates

Depositor

Mark Leake

Insert

Carboxypeptidase Y (PRC1 Budding Yeast)

Tags

mScarletI

Expression

Yeast

Mutation

Deletion signal peptide, G255A mutation

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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CUP1-∆ssCPY*-mNeonGreen

Plasmid

#221156

Purpose

Fluorescent reporter for cytoplasmic aggregates

Depositor

Mark Leake

Insert

Carboxypeptidase Y (PRC1 Budding Yeast)

Tags

mNeonGreen

Expression

Yeast

Mutation

Deletion signal peptide, G255A mutation

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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CUP1-∆ssCPY*-mNeonGreen in pRS315

Plasmid

#221160

Purpose

Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

Depositor

Mark Leake

Insert

Carboxypeptidase Y (PRC1 Budding Yeast)

Tags

mNeonGreen

Expression

Yeast

Mutation

Deletion signal peptide, G255A mutation

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM034_P7_3"Lys3

Plasmid

#216459

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41H

Plasmid

#194526

Purpose

Low copy vector backbone with hygromycin B selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42H

Plasmid

#194527

Purpose

High copy vector backbone with hygromycin B selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41K-TDH3pr

Plasmid

#194528

Purpose

Low copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42K-TDH3pr

Plasmid

#194529

Purpose

High copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41N-TDH3pr

Plasmid

#194530

Purpose

Low copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41H-TDH3pr

Plasmid

#194532

Purpose

Low copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41K-TDH3pr-mNeon

Plasmid

#194534

Purpose

Low copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

kanMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42K-TDH3pr-mNeon

Plasmid

#194535

Purpose

High copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

kanMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41N-TDH3pr-mNeon

Plasmid

#194536

Purpose

Low copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

natAC

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge