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pRS316-SEC18-L1374

Plasmid

#226275

Purpose

Plasmid expressing the SEC18 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-S288C

Plasmid

#226274

Purpose

Plasmid expressing the SEC18 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-L1374

Plasmid

#226271

Purpose

Plasmid expressing the SEC22 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS315-SEC22-UWOPS872421

Plasmid

#226272

Purpose

Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS316-SEC18-NCYC110

Plasmid

#226277

Purpose

Plasmid expressing the SEC18 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS316-SEC18-UWOPS872421

Plasmid

#226276

Purpose

Plasmid expressing the SEC18 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS315-SEC22-S288C

Plasmid

#226270

Purpose

Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS313-SCT1-L1374

Plasmid

#226267

Purpose

Plasmid expressing the SCT1 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM038_P8b_5'Lys3

Plasmid

#216463

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pML107-LUG1

Plasmid

#226265

Purpose

Plasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 gene

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LUG1 gRNA (YLR352W Budding Yeast)

Use

CRISPR

Expression

Yeast

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS316-SSD1-NCYC110

Plasmid

#226279

Purpose

Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pML104-LEU2-2

Plasmid

#226263

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS313-SCT1-NCYC110

Plasmid

#226269

Purpose

Plasmid expressing the SCT1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS313-SCT1-UWOPS872421

Plasmid

#226268

Purpose

Plasmid expressing the SCT1 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Publication

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJRoC30_Th3.1

Plasmid

#188065

Purpose

FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)

Depositor

Sarel Fleishman

Publication

Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

Insert

FuncLib-designed high-redox potential laccase from Trametes hirsuta

Tags

S. cerevisiae evolved α-factor prepro-leader sign…

Expression

Yeast

Mutation

20 PROSS + 4 FuncLib mutations, described in publ…

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJRoC30_Th3.7

Plasmid

#188066

Purpose

FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.7)

Depositor

Sarel Fleishman

Publication

Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

Insert

FuncLib-designed high-redox potential laccase from Trametes hirsuta

Tags

S. cerevisiae evolved α-factor prepro-leader sign…

Expression

Yeast

Mutation

20 PROSS + 3 FuncLib mutations, described in publ…

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJRoC30_Th3.10

Plasmid

#188067

Purpose

FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)

Depositor

Sarel Fleishman

Publication

Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

Insert

FuncLib-designed high-redox potential laccase from Trametes hirsuta

Tags

S. cerevisiae evolved α-factor prepro-leader sign…

Expression

Yeast

Mutation

20 PROSS + 3 FuncLib mutations, described in publ…

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYAMTrGCas

Plasmid

#224866

Purpose

Yeast genome editing

Depositor

Katsunori Suzuki

Publication

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYAMTr2GCsgSeLEU2

Plasmid

#224870

Purpose

Disruption of S. eubayanus type LEU2 genes

Depositor

Katsunori Suzuki

Publication

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYAMTrGCsgScLEU2

Plasmid

#224869

Purpose

Disruption of S. cerevisiae type LEU2 genes

Depositor

Katsunori Suzuki

Publication

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge