8,126 results

1. pOAD eIF4G1 (1-341)

   Plasmid

   #196833

   Purpose

   Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.

   Depositor

   Stan Fields

   Article

   Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

   Insert

   eIF4G1 (TIF4631 Budding Yeast)
   

   Tags

   Gal4

   Expression

   Yeast

   Mutation

   amino acids 1-341

   Promoter

   ADH

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACT2-μ1A

   Plasmid

   #198168

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-1 μ1A
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pACT2-μ2

   Plasmid

   #198173

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-2 μ2
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGBT9-AAGAB

   Plasmid

   #198332

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AAGAB (AAGAB Human)
   

   Tags

   GAL4-DNA binding domain (BD) fragment

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOBD2 Pab1 WT (123-204)

   Plasmid

   #196834

   Purpose

   Expresses Pab1 RRM2 fragment fused to GAL4 DNA binding domain

   Depositor

   Stan Fields

   Article

   Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

   Insert

   Pab1 (PAB1 Budding Yeast)
   

   Tags

   Gal4

   Expression

   Yeast

   Mutation

   123-204 amino acids

   Promoter

   ADH

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOBD2 Pab1 E181R (123-204)

   Plasmid

   #196835

   Purpose

   Expresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain

   Depositor

   Stan Fields

   Article

   Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

   Insert

   Pab1 (PAB1 Budding Yeast)
   

   Tags

   Gal4

   Expression

   Yeast

   Mutation

   123-204 amino acids & E181R

   Promoter

   ADH

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pROS13-Tps2/Gsy1

   Plasmid

   #196613

   Purpose

   Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   S000001911 (TPS2, GSY1 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGADT7-γ1

   Plasmid

   #197078

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-1 γ1 (Ap1g1 Mouse)
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGADT7-ε

   Plasmid

   #197261

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-4 ε (AP4E1 Human)
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   silent substitutions in codons 905, 1129 and 1137

   Promoter

   ADH1

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBT9-tepsin

    Plasmid

    #196647

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-tepsin fusion protein

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    tepsin (TEPSIN Human)
    

    Use

    Yeast two-hybrid vector

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    silent substitution in codon 199 to eliminate int…

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GA46

    Plasmid

    #196614

    Purpose

    Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
    

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUGP1.1

    Plasmid

    #196615

    Purpose

    Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
    

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Promoter

    Native promoter

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. UG75-PAH1-3HA

    Plasmid

    #184761

    Purpose

    PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    PAH1 (PAH1 Budding Yeast)
    

    Tags

    3HA

    Expression

    Yeast

    Promoter

    PAH1

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. UG75-CDS1-3HA

    Plasmid

    #184760

    Purpose

    CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    CDS1 (CDS1 Budding Yeast)
    

    Tags

    3HA

    Expression

    Yeast

    Promoter

    CDS1

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. UG75-DGK1-3HA

    Plasmid

    #184759

    Purpose

    DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    DGK1 (DGK1 Budding Yeast)
    

    Tags

    3HA

    Expression

    Yeast

    Promoter

    DGK1

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAS-mak10 gRNA-MAK10Δ

    Plasmid

    #160384

    Purpose

    For CRISPR-mediated deletin og MAK10 in yeast

    Depositor

    John McCusker

    Insert

    MAK10
    

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAS-mak10 gRNA

    Plasmid

    #160385

    Purpose

    Expresses guide RNA for targeting MAK10 in yeast

    Depositor

    John McCusker

    Insert

    mak10 gRNA
    

    Expression

    Yeast

    Promoter

    RNA pol III promoter (tRNA-Tyr)

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OM14-2EGFP-UG74-ter

    Plasmid

    #184758

    Purpose

    Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    OM14 (OM14 Budding Yeast)
    

    Tags

    2EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SEC7-2EGFP-ter-NAT

    Plasmid

    #184764

    Purpose

    Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    SEC7 (SEC7 Budding Yeast)
    

    Tags

    2EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CIT1-2EGFP-ter-URA3

    Plasmid

    #184767

    Purpose

    Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    CIT1 (CIT1 Budding Yeast)
    

    Tags

    2EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge