8,060 results

1. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

   Plasmid

   #91806

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Residues A1529-G1534 replaced with a PreScission …

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

   Plasmid

   #91807

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   PreScission Protease site (LEVLFQGP) inserted bet…

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CPH3486 (YCp LEU2 Rpb1 F1492A)

   Plasmid

   #91808

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed phenylalanine 1492 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CPH3487 (YCp LEU2 Rpb1 S1493A)

   Plasmid

   #91809

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed serine 1493 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CPH3488 (YCp LEU2 Rpb1 P1494A)

   Plasmid

   #91810

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a P1494A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed proline 1494 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CPH3508 (YCp LEU2 Rpb1 Y1473A)

   Plasmid

   #91811

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a Y1473A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed tyrosine 1473 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CPH3509 (YCp LEU2 Rpb1 T1471A)

   Plasmid

   #91812

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed threonine 1471 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CPH3511 (YCp LEU2 Rpb1 P1472A)

   Plasmid

   #91813

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed proline 1472 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVG50

   Plasmid

   #164708

   Purpose

   yEVenus under the control of tetOpr with ADH1t upstream of the reporter

   Depositor

   Sahand Jamal Rahi

   Article

   Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

   Insert

   ADH1t - tetOpr - yEVenus - CLN2 PEST - ADH1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVG88

    Plasmid

    #164709

    Purpose

    yEVenus under the control of ARG3 promoter

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    ARG3pr - yEVenus - CLN2 PEST - ADH1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YIplac211-KAR2-mEGFP-HDEL

    Plasmid

    #160467

    Purpose

    Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signal

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    KAR2-mEGFP-HDEL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCL10

    Plasmid

    #164710

    Purpose

    yEVenus under the control of MET3 promoter

    Depositor

    Fred Cross

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    MET3pr - yEVenus - CLN2 PEST - ADH1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19-PpSEC13-mEGFP

    Plasmid

    #160469

    Purpose

    P. pastoris integration vector for the gene replacement of SEC13 fused to mEGFP

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    ppSEC13-mEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-PpSEC13-msGFP2

    Plasmid

    #160470

    Purpose

    P. pastoris integration vector for the gene replacement of SEC13 fused to msGFP2

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    ppSEC13-msGFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Dcp2 ΔN

    Plasmid

    #163575

    Purpose

    express wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiae

    Depositor

    Roy Parker

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of N-terminal domain (1-242) of Dcp2

    Promoter

    DCP2 promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Dcp2 300

    Plasmid

    #163577

    Purpose

    express Dcp2 300 at endogenous level in S.cerevisiae

    Depositor

    Roy Parker

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of C-terminal domian (301-970)

    Promoter

    DCP2 promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNTI763 p(Z)-mCherry + UBC9 sgRNA

    Plasmid

    #164918

    Purpose

    CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    P(Z)-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P(Z), synthetic promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

    Plasmid

    #164916

    Purpose

    Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    GCN4(UTR)-ZEM(TF)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1 (yeast)

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNTI743 dual-barcoded gRNA parent vector

    Plasmid

    #164915

    Purpose

    CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNTI733 pRPR1(TetO)-RPC31-sgRNA

    Plasmid

    #164913

    Purpose

    For yeast genomic integration of sgRNA against RPC31

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    pRPR1(TetO)-RPC31-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RPR1

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge