8,173 results
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Plasmid#158137PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the third serine residue…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p-FAPB2.3.6L1TAG
Plasmid#158143PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertFAPB2.3.6L1TAG
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165V
Plasmid#158965PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165V
ExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165R
Plasmid#158966PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165R
ExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFapO_94
Plasmid#149597PurposepXI-3-LoxP-KlURA3-pTEF1- FapO_94-yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterTEF1-FapO_94Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXylO_94
Plasmid#149598PurposepXI - 3 -LoxP -KlURA3 -pTEF1 - XylO_94 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterTEF1-XylO_94Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetO_105
Plasmid#149599PurposepXI - 3 -LoxP -KlURA3 -pTEF1 - TetO_105 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterTEF1-TetO_105Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA271
Plasmid#149600Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_183 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastAvailable SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA272
Plasmid#149601Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_182 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastAvailable SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA197
Plasmid#149602Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterPcaO_1O8Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVD_79
Plasmid#149603Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - TetRDepositorInsertTetR
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyExpressionYeastPromoteract1Available SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL27-decoder
Plasmid#159449PurposeInsert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPfus1tet-citrine-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1tetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1tetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVD_01
Plasmid#149606PurposepTEF1-VanR inserted in pRS416 backboneDepositorInsertVanR
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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