8,128 results

1. pPICZ-His6-K2P4.1B-mCherry-StreptagII

   Plasmid

   #158744

   Purpose

   Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

   Depositor

   Arthur Laganowsky

   Article

   Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

   Insert

   K2P4.1-B (KCNK4 Human)
   

   Tags

   His6 and mCherry with Streptag II

   Expression

   Yeast

   Mutation

   Residues 1-290 and N-linked glycosylation sites r…

   Promoter

   AOX1

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p415 Glo3 S389,398 A

   Plasmid

   #129485

   Purpose

   Expression in S. cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 S389,398 A (GLO3 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   Mutation of serines 389 and 398 to alanine

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p415 Glo3 S389,398 D

   Plasmid

   #129486

   Purpose

   Expression in S. cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 S389,398 D (GLO3 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   Mutation of serines 389 and 398 to Asp

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p415 Met25 Glo3 ΔGRM-ΔC

   Plasmid

   #129487

   Purpose

   Expression in S. cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 ΔGRM ΔC (GLO3 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   Δ376-493 (deletion of Glo3 motif & C-terminal…

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p415 Glo3 R59K

   Plasmid

   #112652

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 R59K
   

   Expression

   Yeast

   Mutation

   R59K (GAP-dead)

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p415 Glo3 R59K ΔC

   Plasmid

   #112653

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 R59K ΔC
   

   Expression

   Yeast

   Mutation

   R59K, Δ431-493 (deletion of C-terminal amphipathi…

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p415 Glo3 R59K ΔGRM-ΔC

   Plasmid

   #112654

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 R59K ΔGRM ΔC
   

   Expression

   Yeast

   Mutation

   R59K, Δ376-493 (deletion of Glo3 motif & C-te…

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p415 Glo3 R59K Δ2x+ve

   Plasmid

   #112655

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 R59K Δ2x+ve
   

   Expression

   Yeast

   Mutation

   R59K, mutations (R223E, K224E, K225E, K233E, K234…

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p415 Glo3 R59K S389,398 A

   Plasmid

   #112656

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 R59K S389,398A
   

   Expression

   Yeast

   Mutation

   R59K, mutations (S389A, S398A) in Glo3 motif, non…

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p415 Glo3 R59K S389,398 D

    Plasmid

    #112657

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 R59K S389,398D
    

    Expression

    Yeast

    Mutation

    R59K, mutations (S389D, S398D) in Glo3 motif, pho…

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p415 GFP-Glo3 ΔGRM-ΔC

    Plasmid

    #112659

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP-Glo3 ΔGRM ΔC
    

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Δ376-493 (deletion of Glo3 motif & C-terminal…

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p415 GFP-Glo3 S389,398 A

    Plasmid

    #112660

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP- Glo3 S389,398A
    

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    mutations (S389A, S398A) in Glo3 motif, non-phosp…

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p415 GFP-Glo3 S389,398 D

    Plasmid

    #112661

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP-Glo3 S389,398D
    

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    mutations (S389D, S398D) in Glo3 motif, phosphomi…

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p415 GFP-Glo3 Δ2x+ve

    Plasmid

    #112662

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP- Glo3 Δ2x+ve
    

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Mutations (R223E, K224E, K225E, K233E, K234E, K23…

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p415 Gcs1 R54K L246D

    Plasmid

    #112648

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Gcs1 R54K L246D
    

    Expression

    Yeast

    Mutation

    R54K & L246D (in ALPS motif)

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p415 Gcs1 R54K AxxA

    Plasmid

    #112649

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Gcs1 R54K AxxA
    

    Expression

    Yeast

    Mutation

    R54K & Alanine substitution of C-terminal δL …

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p415 Gcs1 Δ3xF AxxA

    Plasmid

    #112650

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Gcs1 Δ3xF AxxA
    

    Expression

    Yeast

    Mutation

    R54K, W304A, W307A, W310A, W349A, F353A

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p415 Gcs1 R54K

    Plasmid

    #112647

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Gcs1 R54K
    

    Expression

    Yeast

    Mutation

    R54K

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT140_GalL_cCDA1-VRERBE3

    Plasmid

    #145103

    Purpose

    Expressing base editor cCDA1-VRERBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-VRERBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT46_GalL_cCDA1-BE3

    Plasmid

    #145039

    Purpose

    Expresses cCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    cCDA1-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge