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pRS416-HNT2_intron

Plasmid

#127603

Purpose

low-copy URA-selectable plasmid, HNT2 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

HNT2 intron

Expression

Yeast

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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p415:iCas9

Plasmid

#138266

Purpose

Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

mTN3-GGS6-dCas9

Use

CRISPR

Expression

Yeast

Mutation

Cas9-D10A, H840A

Promoter

GalL

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(G:H)

Plasmid

#138257

Purpose

Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

GFP targeting sgRNAs G and H

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(NT)

Plasmid

#138258

Purpose

Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-ysg(NT)

Plasmid

#138263

Purpose

Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMV23

Plasmid

#128887

Purpose

Position 4 module- STOP (stop codon)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

STOP

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMV16

Plasmid

#128894

Purpose

Position 6 module- LEU2

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

LEU2

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMV47

Plasmid

#128866

Purpose

Position 1 module- IX301997 (yeast integrative region IX301997)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Yeast integrative region chrIX301997

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMV22

Plasmid

#128884

Purpose

Position 3 module- Venus

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Venus

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMV42

Plasmid

#128888

Purpose

Position 4 module- mCherry-STOP

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

mCherry+STOP

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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S3_007_026_MV1

Plasmid

#134419

Purpose

pYTKMV1-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_008_026_MV1

Plasmid

#134420

Purpose

pYTKMV1-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pRPL18B

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_009_026_MV1

Plasmid

#134421

Purpose

pYTKMV1-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_007_026_MV2

Plasmid

#134424

Purpose

pYTKMV2-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_007_026_096

Plasmid

#134410

Purpose

pYTK096-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pTDH3

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_008_026_096

Plasmid

#134411

Purpose

pYTK096-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pRPL18B

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMM0649

Plasmid

#129010

Purpose

Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1

Expression

Yeast

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pYES2-STE2-1D4

Plasmid

#133424

Purpose

Mutant yeast STE2 coding sequence in vector for expression in yeast S. cerevisiae, with Gal1 promoter.

Depositor

Gerhard Wagner

Insert

STE2 (STE2 Budding Yeast)

Expression

Yeast

Mutation

C59S, C252A

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAWH-IRP

Plasmid

#133896

Purpose

Positive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

IRP

Expression

Yeast

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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S3_009_026_096

Plasmid

#134412

Purpose

pYTK096-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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