8,133 results

1. pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry

   Plasmid

   #68276

   Purpose

   SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Ste7DS NLS(4E) mCherry
   

   Tags

   mCherry

   Expression

   Yeast

   Promoter

   RPS2

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB2336

   Plasmid

   #67541

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK275

   Plasmid

   #73363

   Purpose

   Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)

   Depositor

   T. J. Kappock

   Insert

   delta-8 sphingolipid desaturase
   

   Expression

   Yeast

   Mutation

   missing residues 1-6

   Promoter

   GAL1

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK138

   Plasmid

   #73314

   Purpose

   Pas2p peroxisomal membrane targeting sequence

   Depositor

   T. J. Kappock

   Insert

   Pas2p (peroxisomal membrane targeting region)
   

   Tags

   His6 and c-myc

   Expression

   Yeast

   Mutation

   encodes residues 1-42

   Promoter

   AOX1

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK141

   Plasmid

   #73316

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag

   Depositor

   T. J. Kappock

   Insert

   delta-12 fatty acid acetylenase
   

   Tags

   His6, Pas2p signal sequence, and c-myc

   Expression

   Yeast

   Mutation

   synthetic gene

   Promoter

   AOX1

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK137

   Plasmid

   #73313

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tag

   Depositor

   T. J. Kappock

   Insert

   delta-12 fatty acid acetylenase
   

   Tags

   His6 and c-myc

   Expression

   Yeast

   Mutation

   synthetic gene

   Promoter

   5'-AOX1

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTD125 GFP:POP1

   Plasmid

   #71387

   Purpose

   localization of yeast RNase POP1 (RNase MRP binding protein) in yeast

   Depositor

   Mark Schmitt

   Article

   Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

   Insert

   POP1 (POP1 Budding Yeast)
   

   Tags

   GFP

   Expression

   Yeast

   Promoter

   ACT1

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pUG35-YDL183c-GFP

   Plasmid

   #70161

   Purpose

   yeast expression of YDL183c with GFP fusion

   Depositor

   Karin Nowikovsky

   Article

   Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

   Insert

   YDL183c
   

   Tags

   yEGFP

   Expression

   Yeast

   Promoter

   MET17

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

9. YCp33-YDL183c-HA

   Plasmid

   #70164

   Purpose

   yeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal end

   Depositor

   Karin Nowikovsky

   Article

   Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

   Insert

   YDL183c
   

   Tags

   3xHA

   Expression

   Yeast

   Promoter

   YDL183c (217 nucleotides upstream of the ATG)

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pJK349

    Plasmid

    #71566

    Purpose

    Produces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta12 fatty acid acetylenase
    

    Expression

    Bacterial and Yeast

    Promoter

    GAL1

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDS2-aurZ

    Plasmid

    #70151

    Purpose

    Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    aurZ
    

    Expression

    Yeast

    Promoter

    Tef

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMES140

    Plasmid

    #71849

    Purpose

    Complementation of NME1 gene disruption or mutation in yeast

    Depositor

    David Clayton, Mark Schmitt

    Article

    Schmitt et al Mol Cell Biol. 1993 Dec;13(12):7935-41.

    Insert

    NME1 (NME1 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    NME1

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK274

    Plasmid

    #71564

    Purpose

    Produces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta12 fatty acid desaturase (form 3)
    

    Expression

    Bacterial and Yeast

    Promoter

    GAL1 promoter

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK272

    Plasmid

    #71562

    Purpose

    Produces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta12 fatty acid acetylenase
    

    Expression

    Bacterial and Yeast

    Promoter

    GAL1

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDS3-aurJ

    Plasmid

    #70152

    Purpose

    Fusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    aurJ
    

    Expression

    Yeast

    Promoter

    GPD1

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pDS3-X4

    Plasmid

    #70140

    Purpose

    Yeast chr. integration vector with DS3 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    DS3
    

    Expression

    Yeast

    Promoter

    AgTEF1

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS425-M25-Afg1

    Plasmid

    #61720

    Purpose

    yeast Afg1 AAA protein in yeast expression vector

    Depositor

    Oleh Khalimonchuk

    Article

    Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

    Insert

    Afg1 (AFG1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pRS415-Cox15-13xMyc

    Plasmid

    #61721

    Purpose

    yeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vector

    Depositor

    Oleh Khalimonchuk

    Article

    Swenson et al J Biol Chem. 2016 Mar 3. pii: jbc.M115.7

    Insert

    Cox15 (COX15 Budding Yeast)
    

    Tags

    13xMyc

    Expression

    Yeast

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCfB2229

    Plasmid

    #67537

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. LD123

    Plasmid

    #69173

    Purpose

    Integration of GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg23-EGFP
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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