8,133 results

1. LD114

   Plasmid

   #69167

   Purpose

   Integration of GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg14-EGFP
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LD105

   Plasmid

   #69162

   Purpose

   Integration of GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg5-EGFP
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BS-Trp1

   Plasmid

   #69196

   Purpose

   complementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used.

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   TRP1
   

   Expression

   Yeast

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCyc-Bem1

   Plasmid

   #66573

   Purpose

   Express an additional copy of Bem1, integrated at TRP locus

   Depositor

   Orion Weiner

   Article

   Jost et al ACS Synth Biol. 2015 Jun 2.

   Insert

   Bem1
   

   Expression

   Yeast

   Mutation

   F99I*(see below)

   Promoter

   pCyc1

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LD131

   Plasmid

   #69177

   Purpose

   Integration of GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg31-EGFP
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LD129

   Plasmid

   #69176

   Purpose

   Integration of GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg29-EGFP
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LD124

   Plasmid

   #69174

   Purpose

   Integration of GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg24-EGFP
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LD120

   Plasmid

   #69171

   Purpose

   Integration of GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg20-EGFP
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pED55 pRS306 pRPS2-SteND-NLS-mCherry

   Plasmid

   #68277

   Purpose

   SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Ste7ND NLS mCherry
   

   Tags

   mCherry

   Expression

   Yeast

   Promoter

   pRPS2

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

    Plasmid

    #68275

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS(4A) mCherry
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    pRPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCyc-Cdc24

    Plasmid

    #66574

    Purpose

    Express an additional copy of Cdc24, integrated at TRP locus

    Depositor

    Orion Weiner

    Article

    Jost et al ACS Synth Biol. 2015 Jun 2.

    Insert

    Cdc24
    

    Expression

    Yeast

    Promoter

    pCyc1

    Available Since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRPR1_g2gRNA_RPR1t

    Plasmid

    #64388

    Purpose

    encodes g2 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    g2 gRNA
    

    Expression

    Yeast

    Promoter

    pRPR1

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pRS424-TUB2 - human TUBB3 Cterminal tail delta K

    Plasmid

    #60404

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available Since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pRS424-TUB2 - human TUBB1 Cterminal tail

    Plasmid

    #60400

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available Since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pRS424-TUB2 - human TUBB3 Cterminal tail

    Plasmid

    #60397

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Expression

    Yeast

    Mutation

    C127S; TUB2 amino acids 429 - end, replaced with …

    Promoter

    GAL

    Available Since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pRPR1_c2gRNA_RPR1t

    Plasmid

    #64380

    Purpose

    encodes c2 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c2 gRNA
    

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pRPR1_g1gRNA_RPR1t

    Plasmid

    #64387

    Purpose

    encodes g1 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    g1 gRNA
    

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pRPR1_c7gRNA_RPR1t

    Plasmid

    #64385

    Purpose

    encodes c7 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c7 gRNA
    

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pRPR1_c1gRNA_RPR1t

    Plasmid

    #64379

    Purpose

    encodes c1 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c1 gRNA
    

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pRPR1_c5gRNA_RPR1t

    Plasmid

    #64383

    Purpose

    encodes c5 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c5 gRNA
    

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only