8,418 results
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Plasmid#61720Purposeyeast Afg1 AAA protein in yeast expression vectorDepositorInsertAfg1 (AFG1 Budding Yeast)
ExpressionYeastAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS415-Cox15-13xMyc
Plasmid#61721Purposeyeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vectorDepositorAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2229
Plasmid#67537PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD123
Plasmid#69173PurposeIntegration of GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg23-EGFP
TagsEGFPExpressionYeastAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD114
Plasmid#69167PurposeIntegration of GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg14-EGFP
TagsEGFPExpressionYeastAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD105
Plasmid#69162PurposeIntegration of GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg5-EGFP
TagsEGFPExpressionYeastAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
BS-Trp1
Plasmid#69196Purposecomplementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used.DepositorInsertTRP1
ExpressionYeastAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCyc-Bem1
Plasmid#66573PurposeExpress an additional copy of Bem1, integrated at TRP locusDepositorPublicationInsertBem1
ExpressionYeastMutationF99I*(see below)PromoterpCyc1Available sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD-BS-Trp1
Plasmid#69503PurposeImproved version for complementation of yeast auxotrophic marker TRP1DepositorInsertTRP1
ExpressionYeastAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD131
Plasmid#69177PurposeIntegration of GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg31-EGFP
TagsEGFPExpressionYeastAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD129
Plasmid#69176PurposeIntegration of GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg29-EGFP
TagsEGFPExpressionYeastAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD124
Plasmid#69174PurposeIntegration of GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg24-EGFP
TagsEGFPExpressionYeastAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD120
Plasmid#69171PurposeIntegration of GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg20-EGFP
TagsEGFPExpressionYeastAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED55 pRS306 pRPS2-SteND-NLS-mCherry
Plasmid#68277PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutationDepositorInsertSte7ND NLS mCherry
TagsmCherryExpressionYeastPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry
Plasmid#68275PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLSDepositorInsertSte7DS NLS(4A) mCherry
TagsmCherryExpressionYeastPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCyc-Cdc24
Plasmid#66574PurposeExpress an additional copy of Cdc24, integrated at TRP locusDepositorPublicationInsertCdc24
ExpressionYeastPromoterpCyc1Available sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_g2gRNA_RPR1t
Plasmid#64388Purposeencodes g2 gRNADepositorPublicationInsertg2 gRNA
ExpressionYeastPromoterpRPR1Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB3 Cterminal tail delta K
Plasmid#60404PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPKDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB1 Cterminal tail
Plasmid#60400PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB3 Cterminal tail
Plasmid#60397PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationC127S; TUB2 amino acids 429 - end, replaced with …PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only