8,133 results

1. pHCMM3

   Plasmid

   #167454

   Purpose

   A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

   Depositor

   Hugo Cesar Medina-Munoz, Marvin Wickens

   Article

   Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   PSEC63_yeGFP_cePUP-2_URA3_TADH1
   

   Expression

   Yeast

   Promoter

   PSEC63

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAV10.F3.loxP

   Plasmid

   #63200

   Purpose

   pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAV10.K3.loxP

   Plasmid

   #63202

   Purpose

   pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAV10.K6.loxP

   Plasmid

   #63203

   Purpose

   pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV10.K5.loxP

   Plasmid

   #63204

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV10.HO3.loxP

   Plasmid

   #63205

   Purpose

   pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV10.HO5.loxP

   Plasmid

   #63206

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV10.HO6

   Plasmid

   #63211

   Purpose

   pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. yFusionRed

   Plasmid

   #111916

   Purpose

   yeast optimized FusionRed

   Depositor

   Patrick Lajoie

   Article

   Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

   Insert

   yFusionRed
   

   Expression

   Yeast

   Promoter

   GPD

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJW1668

    Plasmid

    #112042

    Purpose

    CEN/ARS plasmid for GAL-inducible FET3-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    FET3
    

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Promoter

    GAL1

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJW1669

    Plasmid

    #112043

    Purpose

    CEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    NSG2
    

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Promoter

    GAL1

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJW1672

    Plasmid

    #112046

    Purpose

    CEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    PST1
    

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Promoter

    GAL1

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJW1674

    Plasmid

    #112048

    Purpose

    CEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    SWP1
    

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Promoter

    GAL1

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge