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Plasmid#158126PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertmTagBFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDS190
Plasmid#34977DepositorInsertePDZb
UseTagsExpressionYeastMutationPromoterADHAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoSuperfolderGFP-Kan
Plasmid#44901PurposeYeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with Kan selectionDepositorInsertyoSuperfolderGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYSD300
Plasmid#212930PurposeEncodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.DepositorInsertAGA1 (AGA1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
PHBA02
Plasmid#83536PurposeConstitutive TEF1 promoter mediated expression of the UBiC and ARO4* genesDepositorInsertpTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS267
Plasmid#122380PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDZ276 (pURA MET28 PCP-2x-yeGFP)
Plasmid#35194DepositorInsertPCP-2x-yeGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
PP244
Plasmid#78978PurposePromoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
FRP2371
Plasmid#127577PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT
Plasmid#140594PurposeExpression in S. cerevisiaeDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK1
Plasmid#111722Purposeto express human BLM protein in yeast cellsDepositorInsertBLM (BLM Human)
UseTagshexahistidineExpressionYeastMutationa C-terminal hexahistidine tag was added, a yeast…PromoterGAL1Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNB2289
Plasmid#113396PurposeGalactose-inducible red fluorescent protein (RFP) codon optimized for S. cerevisiaeDepositorInsertRed fluorescent protein, codon optimized for S. cerevisiae
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pYeDP60-hATP13A2-WT
Plasmid#171377PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
UseTagsBAD tagExpressionYeastMutationPromoterURA3Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA-leu-HYB
Plasmid#64332Purposeleu2 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of leu2 deletion gRNA cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G1-Ph
Plasmid#46814PurposeTEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRG659_lexO-FseI_LexA-TF_LEU2MX
Plasmid#154816PurposeInducible expression of FseI endonuclease in S. cerevisiaeDepositorInsertsFseI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM119
Plasmid#133661PurposeIntegration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.DepositorInsertp1k-NAB2-mTagBFP2 (NAB2 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-yomRuby3-SpHIS5
Plasmid#204287PurposeFor C-terminal tagging using yeast optimized mRuby3DepositorInsertpFA6-yomRuby3-SpHIS5
UseTagsyomRuby3ExpressionYeastMutationPromoternoneAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymTq2-URA3
Plasmid#168057PurposeReplaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymTurquoise2
UseTagsymTurquoise2ExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only