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pJW1674

Plasmid

#112048

Purpose

CEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Publication

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SWP1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-MET17

Plasmid

#122885

Purpose

ADE2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-LEU2

Plasmid

#122888

Purpose

LYS2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-HIS3

Plasmid

#122891

Purpose

TRP1-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-MET17

Plasmid

#122893

Purpose

TRP1-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-MET17

Plasmid

#122910

Purpose

LYS2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-LEU2

Plasmid

#122913

Purpose

TRP1-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-MET17

Plasmid

#122914

Purpose

TRP1-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-KanMx

Plasmid

#122920

Purpose

LYS2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pEMY01AB-PRO

Plasmid

#127714

Purpose

Integrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Pact1-ITE-Tadh1

Plasmid

#127723

Purpose

Yeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Expression

Yeast

Promoter

Pact1

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Ppre3-ITE-Teno1

Plasmid

#127724

Purpose

Yeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Expression

Yeast

Promoter

Ppre3

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Pact1-ITE-Tdpp1

Plasmid

#127725

Purpose

Yeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Expression

Yeast

Promoter

Pact1

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Phta1-ITE-Tvma2

Plasmid

#127726

Purpose

Yeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Expression

Yeast

Promoter

Phta1

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Prps3-CAD-Tcyc1

Plasmid

#127728

Purpose

Yeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Prps3

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Peft2-CAD-Tklura3

Plasmid

#127729

Purpose

Yeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Peft2

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Psmtef1-CAD-Tyhi9

Plasmid

#127730

Purpose

Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psmtef1

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pfba1p-ACO-Tgpm1

Plasmid

#127731

Purpose

Yeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pykt6-ACO-Tprm9

Plasmid

#127732

Purpose

Yeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pykt6

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Ptef1-ACO-Tyol036w

Plasmid

#127733

Purpose

Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptef1

Availability

Academic Institutions and Nonprofits only

Enlarge