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pPTK021-7-PARS

Plasmid

#84990

Purpose

Type 7, Pichia autonomously replicating sequence

Depositor

Volker Sieber

Publication

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Pichia autonomously replicating sequence

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAG416-Gal-PA50

Plasmid

#84902

Purpose

induce expression of dipeptide repeat PA in yeast

Depositor

Aaron Gitler

Publication

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PA50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEN-L1-K2-L2

Plasmid

#80684

Purpose

Gateway Entry vector containing a temperature-sensitive mouse DHFR

Depositor

Nico Dissmeyer

Publication

Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

Inserts

DHFR (Dhfr Mouse)
DHFR (Dhfr Mustard Weed, Mouse)

Use

Synthetic Biology

Tags

3xHA

Expression

Bacterial, Insect, Mammalia…

Mutation

mouse DHFR with N-terminal Ubiquitin

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCfB2988

Plasmid

#63638

Purpose

pCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKK01

Plasmid

#64744

Purpose

Yeast expression plasmid: phaA, phaB, phaC

Depositor

Jens Nielsen

Publication

Kocharin et al AMB Express. 2012 Sep 25;2(1):52. doi: 1

Insert

phaA, phaB, phaC

Expression

Yeast

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pJZC522

Plasmid

#62279

Purpose

MCP-VP64 fusion

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

MCP-VP64

Tags

VP64

Expression

Yeast

Promoter

pAdh

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC583

Plasmid

#62314

Purpose

sgRNA with 2x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 binding module

Expression

Yeast

Promoter

SNR52

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJZC506

Plasmid

#62281

Purpose

COM-VP64 fusion

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

COM-VP64

Tags

VP64

Expression

Yeast

Promoter

pAdh

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PGAL

Plasmid

#60337

Purpose

Contains full length native galactose promoter

Depositor

Hal Alper

Publication

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Inserts

promoter
yECitrine

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pPOP

Plasmid

#59526

Purpose

Vector for pop-in/pop-out gene replacement in Pichia pastoris.

Depositor

Benjamin Glick

Publication

Soderholm et al Biotechniques. 2001 Aug;31(2):306-10, 31

Type

Empty backbone

Expression

Yeast

Promoter

GAP

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-Opi1 + terminator

Plasmid

#58836

Purpose

Expresses GFP-Tagged Opi1 + Terminator in yeast

Depositor

Tim Levine

Insert

Opi1p (OPI1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

PHO5

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
M3927 ura3::KanMX3 Disruptor Converter

Plasmid

#51682

Purpose

Yeast marker swap plasmid for converting URA3 to KanMX3

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-G11-5FLAG-kanMX6

Plasmid

#49182

Purpose

genomic G11-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

G11-5FLAG

Expression

Yeast

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pYX232-mtGFP

Plasmid

#45052

Depositor

Benedikt Westermann

Publication

Westermann et al Yeast. 2000 Nov;16(15):1421-7.

Insert

mtGFP

Expression

Yeast

Promoter

TPI

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFA6a-link-yomRuby-Kan

Plasmid

#44956

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby

Expression

Yeast

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomWasabi-SpHis5

Plasmid

#44850

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomWasabi

Expression

Yeast

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pTH733-2µ-RLuc/maxCFLuc

Plasmid

#40608

Depositor

Tobias von der Haar

Publication

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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Gal-Rad51

Plasmid

#33230

Depositor

Hannah Klein

Publication

Shah et al Mol Cell. 2010 Sep 24;39(6):862-72.

Insert

Rad51 (RAD51 Budding Yeast)

Expression

Yeast

Promoter

Gal1

Available since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-RRM Mutant-YFP

Plasmid

#29608

Depositor

Aaron Gitler

Publication

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS RRM mutant (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

F305L, F341L, F359L and F368L

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pXP222

Plasmid

#26833

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Publication

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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