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pRR-MR-5Z

Plasmid

#23059

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

MR (NR3C2 Human)

Expression

Yeast

Mutation

N528H, Q814R, H993L

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pDRF1 mBaojin-T2A-mCherry

Plasmid

#219842

Purpose

equimolar expression of mBaojin and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mBaojin-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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bRA66

Plasmid

#100952

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERalpha (ESR1 Human)

Expression

Yeast

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
NM R2E2 GFP

Plasmid

#1208

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 NM domain (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

2 additional copies of second oligopeptide repeat…

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pAV0328

Plasmid

#133489

Purpose

Expression of nucleo-cytoplasmic mCherry

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)

Expression

Yeast

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PGAL

Plasmid

#60337

Purpose

Contains full length native galactose promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Inserts

promoter
yECitrine

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pYZ155

Plasmid

#98411

Purpose

Pombe Expression Vector - nmt1 promoter with Sp.ura4 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKmK.C4

Plasmid

#125062

Purpose

Storage plasmid for K.marxianus ARS

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KmARS2

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pG4

Plasmid

#162605

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRSCupSup35GFP

Plasmid

#1087

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only

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pKmK.T3

Plasmid

#125055

Purpose

Storage plasmid for K.marxianus terminator; type 4 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KMXK_A3020 terminator

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCSJ95

Plasmid

#104343

Purpose

Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

FBP1

Tags

3xFLAG

Expression

Yeast

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pKmK.I2R

Plasmid

#125064

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to chromosome V: 23743..21744

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB2795

Plasmid

#63650

Purpose

pCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pKmK.I2L

Plasmid

#125031

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to chromosome V: 23743..21744

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-GA50

Plasmid

#84907

Purpose

induce expression of dipeptide repeat GA in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

GA50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-TID-FKBP-HA3

Plasmid

#227998

Purpose

Expresses TurboID-FKBP fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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BB3cH_pGAP_23*_pPFK300_Cas9

Plasmid

#104912

Purpose

hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Expression

Yeast

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pVW296

Plasmid

#139496

Purpose

Expression vector for the PP7-mCherry protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 PP7∆FG-mCherry tCYC1

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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