8,350 results
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Plasmid#127579PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-yoHaloTag-TLG1
Plasmid#105277PurposePlasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
TagsyoHaloTagExpressionYeastMutationHaloTag has yeast optimized codonsAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN3
Plasmid#233520PurposeNb Vector Plasmid (under galactose induction), AmpR (E. coli) and HygroR (yeast), Cen6/ARSDepositorTypeEmpty backboneExpressionBacterial and YeastPromoterpGAL1Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-Sir2
Plasmid#51742Purposeoverexpression of Sir2 in S. cerevisiae, HIS3 markerDepositorAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAT253
Plasmid#40943DepositorInsertGFP-LacI**
TagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAS9id
Plasmid#141251PurposeGalactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterGAL1 (Cas9), pSNR52 (gRNA)Available SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXZX351
Plasmid#169454PurposepRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassetteDepositorInsertgRNA for MATα
ExpressionYeastPromoterSNR52Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEasyCas9
Plasmid#184909PurposeExpresses the Streptococcus pyogenes Cas9 proteinDepositorInsertCas9
UseCRISPRExpressionBacterial and YeastAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFS552
Plasmid#242852PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW1 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW1 promoterAvailable SinceSept. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-GL
Plasmid#51225Purposebi-directional expression vector for yeast, galactose inducible, LEU2 selectionDepositorTypeEmpty backboneExpressionYeastPromoterGAL1,10Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB5A
Plasmid#83556PurposeUsed to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.DepositorInsertGAL1 promoter-yEGFP
ExpressionYeastPromoterGAL1Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG2
Plasmid#162603PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
ExpressionYeastAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAS9cd
Plasmid#141252PurposeConstitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterTEF1 (Cas9), pSNR52 (gRNA)Available SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-PKM1
Plasmid#34607DepositorAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
FRP2365
Plasmid#127575PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1
Plasmid#58435PurposeInduces Citrine expression from a promoter containing 8 lexA boxes in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)8-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUDP013
Plasmid#103873PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorphaDepositorInsertHH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
UseCRISPRExpressionYeastPromoterScTDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG425GAL-ccdB-EGFP
Plasmid#14201DepositorTypeEmpty backboneUseGateway CloningTagsEGFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-NF1-GRD
Plasmid#19993DepositorInsertNeurofibromatosis type I (NF1 Human)
ExpressionYeastAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only