8,086 results

1. pDRF1-GW ymYPET

   Plasmid

   #118455

   Purpose

   Overexpression of ymYPET

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymYPET
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   YPET A206K, F208S, E232L, N235D

   Promoter

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pI1-MTU-DO-HPH

   Plasmid

   #160014

   Purpose

   Integrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZJOM113

   Plasmid

   #133666

   Purpose

   Integration of VPS4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPS4-mTagBFP2 (VPS4 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGI3EM20C

   Plasmid

   #135487

   Purpose

   Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr H2B-tdTomato
   

   Use

   Tags

   tdTomato

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ45: pRS426-His-MBP-SpuFz1_Native

   Plasmid

   #205252

   Purpose

   S. cerevisiae expression vector for SpuFz1 native guide

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYES2.1-cerMET8

   Plasmid

   #107439

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET8 (MET8 Budding Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRoC30_Th3.14

   Plasmid

   #188068

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 4 FuncLib mutations, described in publ…

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179068

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid droplet

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-PDR16-Link-Scarlet(rfp)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBK79 pHomL-RPL18Bp-Scarlet(rfp)-Link-YPT7-SSA1t-HomR (AmpR)

   Plasmid

   #179063

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-YPT7, a marker for the vacuole, late endosome

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Scarlet(rfp)-Link-YPT7
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZJOM136

    Plasmid

    #133660

    Purpose

    Integration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ELO3-mTagBFP2 (ELO3 Budding Yeast)
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG426-GAL-Alpha-synuclein-H50Q

    Plasmid

    #181724

    Purpose

    Galactose inducible expression of Alpha-synuclein-H50Q

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H50Q

    Promoter

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDS257

    Plasmid

    #34971

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB4282

    Plasmid

    #98701

    Purpose

    A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    gRNA PCR template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDRF1-GW yosfGFP-T2A-mCherry

    Plasmid

    #118448

    Purpose

    Produces equimolar expression of yeast codon-optimized sfGFP and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yosfGFP-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LD127

    Plasmid

    #69175

    Purpose

    Integration of GFP tag at Atg27 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg27-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHES795

    Plasmid

    #87943

    Purpose

    Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    Zif268 DBD - hER LBD - MSN2 AD (ESR1 Human, Budding Yeast, Synthetic)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 (crippled)

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTUP1

    Plasmid

    #104341

    Purpose

    Express TUP1

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

    Insert

    TUP1 (TUP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB2794

    Plasmid

    #63651

    Purpose

    pCfB2794 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG306GPD-ccdB-DsRed

    Plasmid

    #14356

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

    Plasmid

    #24549

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available Since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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