8,057 results

1. pHS12-mCherry

   Plasmid

   #25444

   Depositor

   Benjamin Glick

   Insert

   mCherry
   

   Use

   Tags

   COX4 Presequence

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB2791

   Plasmid

   #63654

   Purpose

   pCfB2791 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNH605-CCAAT-YFP

   Plasmid

   #172132

   Purpose

   Fluorescent reporter for HAP complex activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xCCAAT-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1 core promotor with 4 tandem repeats of the H…

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGFP-ATG13(315)

   Plasmid

   #122072

   Purpose

   Expressing GFP-Atg13

   Depositor

   Daniel Klionsky

   Article

   Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

   Insert

   ATG13
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG425GPD-ccdB-DsRed

   Plasmid

   #14370

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCTcon2-5INS_EV3

   Plasmid

   #102293

   Purpose

   Yeast Surface Display of 5Ins_16_EV3

   Depositor

   Sarel Fleishman

   Article

   Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

   Insert

   Evolved Insulin binding scFV
   

   Use

   Tags

   3x(G4S) Linker, G3S Linker, HA, and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWS926

   Plasmid

   #123052

   Purpose

   LexA bacterial repressor

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   LexA-NLS [3a]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYM28-BirA*

   Plasmid

   #160290

   Purpose

   Used for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: HIS3

   Depositor

   Martin Ott

   Article

   Salvatori et al Mol Cell. 2020 Feb 20;77(4):887-900.e5.

   Insert

   BirA*
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWL89A mmPing20

   Plasmid

   #145788

   Purpose

   mPing Yeast Transposition Assay

   Depositor

   Charles Nathan Hancock

   Article

   Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

   Insert

   mmPing20
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T162C, T258A, T287C, T300A, T304A, T310A, G372A

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pABY-c46

    Plasmid

    #201774

    Purpose

    Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    NbALFA
    

    Use

    Tags

    40aa Linker, mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYeDP60-hATP13A2-E343A

    Plasmid

    #171378

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Use

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    E343A

    Promoter

    URA3

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG425GPD-ccdB-TAP

    Plasmid

    #14274

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDB4279

    Plasmid

    #98698

    Purpose

    A control plasmid containing the intact bsdMX marker

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPS1739

    Plasmid

    #8922

    Depositor

    Pamela Silver

    Article

    Ferrigno et al EMBO J. 1998 Oct 1. 17(19):5606-14.

    Insert

    HOG1 (HOG1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pFA6a-link-yETSapphire-CaUra3

    Plasmid

    #44650

    Depositor

    Kurt Thorn

    Insert

    Turbo Sapphire
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRG216

    Plasmid

    #64528

    Purpose

    Centromeric E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. dCas9v2

    Plasmid

    #103140

    Purpose

    TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    dCas9-VPR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Removed scaffold gRNA from original plasmid

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pK3FS

    Plasmid

    #85776

    Purpose

    N-ICE plasmid pK3FS

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4046203

    Plasmid

    #145488

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. CuGFP-ATG8(416)

    Plasmid

    #49423

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

    Depositor

    Daniel Klionsky

    Article

    Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only