8,086 results

1. mito-yHS1-M7A

   Plasmid

   #159164

   Purpose

   Mitochondrial labile heme reporter

   Depositor

   Amit Reddi

   Article

   Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

   Insert

   mito-yHS1-M7A
   

   Use

   Tags

   COX4 pre-sequence

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of mito-yHS1…

   Promoter

   GPD

   Available Since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pYES2.1-pomSua1

   Plasmid

   #107446

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Sua1
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   See depositor comments below.

   Promoter

   GAL1

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYES2.1-pomMet14

   Plasmid

   #107451

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Met14 (met14 Fission Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYES2.1-pomSir1

   Plasmid

   #107447

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Sir1 (sir1 Fission Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   H523R, A750T

   Promoter

   GAL1

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYES2.1-pomMet1

   Plasmid

   #107444

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Met1
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. D771 leu2::LYS2 Disruptor Converter

   Plasmid

   #51677

   Purpose

   Yeast marker swap plasmid for converting LEU2 to LYS2

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   LYS2 (LYS2 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT11

   Plasmid

   #98308

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   TRPL3ClLIS1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXP622

   Plasmid

   #26849

   Depositor

   Suzanne Sandmeyer, Nancy DaSilva

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046479

   Plasmid

   #145495

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAG416GPD-EGFP-ccdB

    Plasmid

    #14316

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-SKII-3XHA-HALO-Phleomycin

    Plasmid

    #188930

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-Phleomycin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pV1081

    Plasmid

    #111425

    Purpose

    Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgADE-NT2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB4848

    Plasmid

    #106138

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA CUP1p-NuiHA kanMX

    Plasmid

    #131169

    Purpose

    PCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiae

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Use

    Tags

    Nui-HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG416GPD-ccdB-HA

    Plasmid

    #14244

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG426GPD-EYFP-ccdB

    Plasmid

    #14348

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS416gT-GalL-Cas9

    Plasmid

    #79903

    Purpose

    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

    Depositor

    Ronald Davis

    Article

    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

    Inserts

    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPM1 promoter, GalL, and RPR1 promoter with TetO …

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG416GPD-Cerulean-GOS1

    Plasmid

    #18846

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    GOS1 (GOS1 Budding Yeast)
    

    Use

    Tags

    cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pRS316CUP RNQ CFP

    Plasmid

    #1171

    Depositor

    Susan Lindquist

    Article

    Sondheimer et al Mol Cell 2000 Jan;5(1):163-72.

    Insert

    RNQ1 (RNQ1 Budding Yeast)
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pGAN200

    Plasmid

    #129203

    Purpose

    yTRAP sensor of [PSI+], detects aggregation of Sup35

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35NM (SUP35 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    N terminal and middle domains

    Promoter

    SUP35

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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