9,801 results

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pNIC-HIS6-NUSA-TEV-GG

Plasmid

#215798

Purpose

Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-CT-sfGFP-HN-GG

Plasmid

#215803

Purpose

Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC- AVITAG-CTH10STII- -GG

Plasmid

#215805

Purpose

Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Fusion of Zika NS2B-NS3 proteins

Plasmid

#204815

Purpose

Bacterial expression of a fusion of Zika NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika NS2B-NS3 fusion (orf1ab Zika)

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-LASV-GPC

Plasmid

#215690

Purpose

Produces the Lassa virus glycoprotein

Depositor

Melinda Brindley

Publication

Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

Insert

LASV GP

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_RAB7A:1-207

Plasmid

#211096

Purpose

Mammalian expression of full-length human RAB7A. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAB7A:M1-C207 (RAB7A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_BOP1:1-746

Plasmid

#211005

Purpose

Mammalian expression of full-length humanBOP1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BOP1:M1-T746 (BOP1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_WDR91:1-747

Plasmid

#210996

Purpose

Mammalian expression of full-length human WDR91. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WDR91:M1-A747 (WDR91 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_DCAF1:1-1507

Plasmid

#210972

Purpose

Mammalian expression of full-length human DCAF1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-hSyn-Galpha-i1 ONE-GO

Plasmid

#204333

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Publication

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i1 ONE-GO

Use

Lentiviral

Available since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-hSyn-Galpha-q ONE-GO

Plasmid

#204336

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Publication

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-q ONE-GO

Use

Lentiviral

Available since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-hSyn-Galpha-13 ONE-GO

Plasmid

#204337

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Publication

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-13 ONE-GO (GNA13 Human)

Use

Lentiviral

Available since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-i3 G183S ONE-GO

Plasmid

#204338

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Publication

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3 G183S ONE-GO

Use

Lentiviral

Mutation

G183S mutation in Galpha-i3 (renders it RGS insen…

Available since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGL4.84(hRlucCP/Puro)

Plasmid

#212935

Purpose

RapidResponse(TM) Renilla luciferase vector for investigating regions controlling transcription

Depositor

Pete Stecha

Type

Empty backbone

Use

Luciferase

Mutation

WT

Available since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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qTAG-C-miRFP670nano3-Blast-H3C2

Plasmid

#207784

Purpose

Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-moxGFP-Zeo

Plasmid

#207732

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-3xFLAG-Zeo

Plasmid

#207739

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-V5-Blast

Plasmid

#207719

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-sTagRFP-Puro

Plasmid

#207723

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-3xFLAG

Plasmid

#207706

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge