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MAC-C Sars-CoV-2 NSP12_P314L

Plasmid

#194813

Purpose

Sars-CoV-2 NSP12_P314L in MAC-C destination vector

Depositor

Markku Varjosalo

Publication

Varjosalo et al 10 May 2024, Research Square

Insert

NSP12_P314L (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

P314L

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM54418

Plasmid

#219512

Purpose

Transient expression of Pz DFR

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

Pz DFR

Expression

Plant

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM10764

Plasmid

#219511

Purpose

Transient expression of At F3'H

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At F3'H

Expression

Plant

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM10775

Plasmid

#219510

Purpose

Transient expression of At ANS

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At ANS

Expression

Plant

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4169

Plasmid

#200108

Purpose

Prig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFP

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

snb-1 (snb-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prig-3

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4203

Plasmid

#200160

Purpose

Prig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFP

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

twk-40 (twk-40 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prig-3

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4542

Plasmid

#200779

Purpose

Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GCaMP6

Tags

mNeptune

Expression

Worm

Promoter

Ptwk-40

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH1371

Plasmid

#199631

Purpose

Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GPI

Tags

YFP

Expression

Worm

Promoter

Pregf-1

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-NHSTIIT-GG

Plasmid

#215789

Purpose

Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-TolB-NHSTIIT-GG

Plasmid

#215792

Purpose

Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-PelB-NH10T-AVITAG-GG

Plasmid

#215793

Purpose

Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-MBP-TEV-GG

Plasmid

#215794

Purpose

Cloning of target gene with N terminal MBP tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-HIS10-ZBASIC-TEV-GG

Plasmid

#215797

Purpose

Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-NoTag-GG

Plasmid

#215810

Purpose

Cloning of target gene with no tags

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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Fusion of West Nile NS2B-NS3 proteins

Plasmid

#204796

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR54:1-334

Plasmid

#210931

Purpose

Insect expression of full-length human WDR54. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR54:M1-V334 (WDR54 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SEC31A:1-338

Plasmid

#210925

Purpose

Insect expression of human SEC31A fragment M1 to T338. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SEC31A:M1-T338 (SEC31A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_CORO1C:1-474

Plasmid

#210920

Purpose

Insect expression of full-length human CORO1C. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1C:M1-A474 (CORO1C Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_DDA1:1-102

Plasmid

#210902

Purpose

Insect expression of full-length human DDA1. Untagged.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDA1:M1-T102 (DDA1 Human)

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_CDC20:161-477

Plasmid

#210943

Purpose

Insect expression of human CDC20 fragment S161 to P477. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CDC20:S161-P477 (CDC20 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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