9,747 results
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Plasmid#190824PurposeFor mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.DepositorInsertβ-catenin (CTNNB1 Chicken)
TagsGFPExpressionMammalianMutationS23A, T40A, T41A, T112APromoterCMVAvailable sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-17-6
Plasmid#190827PurposeFor mammalian expression of 17-6, a non-aptamer RNA. Expression is driven by a U6 promoter.DepositorInsert17-6 (Non-aptamer RNA)
ExpressionMammalianPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-T1-U6-AP3
Plasmid#190828PurposeFor mammalian expression of T1 and AP3, the RNA aptamers of ncOGT and GFP, respectively. Expression of each aptamer is driven by a U6 promoter.DepositorInsertsT1 (an RNA aptamer of OGT)
AP3 (an RNA aptamer of GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyExpressionMammalianPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-NL1F35
Plasmid#190830PurposeFor mammalian expression of NL1F35, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.DepositorInsertNL1F35 (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyExpressionMammalianPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-NL1F50
Plasmid#190831PurposeFor mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.DepositorInsertNL1F50 (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyExpressionMammalianPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-3JB1F+2
Plasmid#190833PurposeFor mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyExpressionMammalianPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-3JB1F+6
Plasmid#190835PurposeFor mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1F_T1+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyExpressionMammalianPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL001_Hec1-ms_IgG_HC
Plasmid#193618PurposeHec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.DepositorInsertAnti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)
TagsHis6 and signal peptideExpressionMammalianPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL002_Hec1-ms_IgG_LC
Plasmid#193619PurposeHec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.DepositorInsertAnti-Hec1 light chain (Hec1-ms_IgG_LC)
Tagssignal peptideExpressionMammalianPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pShuttle-PR.Cre
Plasmid#192933PurposepShuttle.Cre encoding sgRNAs targeting Trp53 and Rb1 genesDepositorAvailable sinceDec. 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-ANME2bHR1 MCR-NG
Plasmid#192774PurposeExpresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-ANME1G37 MCR-NG
Plasmid#192773PurposeExpresses NG-tagged ANME1G37 MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-ANME1BS MCR-NG
Plasmid#192772PurposeExpresses NG-tagged ANME1BS MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRmar-NG
Plasmid#192766PurposeExpresses NG-tagged MCRmar in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available sinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4260
Plasmid#191678PurposePtwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarletDepositorInsertGCaMP6s
TagswScarletExpressionWormPromoterPtwk-40Available sinceNov. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pILAC3
Plasmid#185890PurposeIn vivo gene amplification (HapAmp) of lycopene synthetic module at RPL25 locusDepositorInsertsee comments
ExpressionYeastMutationERG20 F96C; Xd.CRtYB E83KAvailable sinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Phage-PGK-FLAG-HA-T(WT)
Plasmid#181737Purposefor lentiviral expression of brachyury ORF in mammalian cellsDepositorInsertT (brachyury)
UseLentiviralMutationa silent mutation in the PAM site of an sgRNAt ta…Available sinceAug. 3, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK1024 (pDS-Def1)
Plasmid#183125PurposeExpresses double-stranded RNA targeting the Def1 gene from Apis melliferaDepositorInsertFragment of Def1 gene
UseSynthetic BiologyAvailable sinceMay 19, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
DCAF1:PBC020-D05:C246337
Plasmid#183974PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertDCAF1:PBC020-D05:C246337 (DCAF1 Human)
UseBaculovirus expresssionTags6xHis-TEV and MHHHHHHSSGRENLYFQGPromoterPolyhedrinAvailable sinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
C22orf4:HPC057-F11:C35657
Plasmid#183977PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorAvailable sinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits