9,747 results

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His-TEV-GB1 Leu5Met

Plasmid

#128686

Purpose

Expresses GB1 Leu5Met mutant

Depositor

Sharon Rozovsky

Publication

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Tags

His

Expression

Bacterial

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Phage-PGK-Flag-HA-FKBP-T(WT)

Plasmid

#181734

Purpose

for lentiviral expression of brachyury ORF in mammalian cells

Depositor

Charles Lin

Publication

Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

Insert

T (brachyury)

Use

Lentiviral

Mutation

a silent mutation in the PAM site of an sgRNAt ta…

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5a1_Ptet

Plasmid

#179339

Purpose

gRNA Position Vector 5a1, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5b1_Ptet

Plasmid

#179341

Purpose

gRNA Position Vector 5b1, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5a2_Ptet

Plasmid

#179340

Purpose

gRNA Position Vector 5a2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2198

Plasmid

#179773

Purpose

Prgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFP

Depositor

Mei Zhen

Publication

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::GFP (nlf-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-RiboJ-LacZa-T7

Plasmid

#173224

Purpose

pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems

Depositor

Jim Haseloff

Publication

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH3456

Plasmid

#179581

Purpose

Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Publication

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

BFP

Expression

Worm

Promoter

Pnmr-1

Available since

March 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH3449

Plasmid

#179576

Purpose

Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Publication

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

BFP

Expression

Worm

Promoter

Plgc-55B

Available since

March 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_5a6.10

Plasmid

#178934

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CDS_aadA_chlo

Plasmid

#163935

Purpose

L0 part - CDS

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

aadA gene for spectinomycin resistance

Expression

Bacterial

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3TERM_bba_b0015_rev_chlo

Plasmid

#163931

Purpose

L0 part - terminator

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3, prokaryotic double terminator BBa_B0015

Expression

Bacterial

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:mS-Lt-Ck4

Plasmid

#136132

Purpose

L1 in position 4, mark cell outline with mScarletI

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mScarletI-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_35Sx2

Plasmid

#136112

Purpose

Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_35Sx2 (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3TERM_Nos

Plasmid

#136115

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_Nos (A. tumefaciens)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_KanR-Ck1

Plasmid

#136123

Purpose

L1 in position 1 (next to LB in L2 construct), for KanR

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:nptII_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_linker-mTurquoise2

Plasmid

#136100

Purpose

FP for C-term fusion with CDS12 without linker

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAlaGlySer_mTurquoise2

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_35S

Plasmid

#136111

Purpose

Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsC_spacer

Plasmid

#136077

Purpose

Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE_spacer

Plasmid

#136078

Purpose

Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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