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pJH4018

Plasmid

#200043

Purpose

Prig-3 FRT let858 (stop) FRT zif-1 SL2 EBFP unc-54 3' UTR C.elegans AVA and other neurons expression of zif EBFP

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

zif-1 (zif-1 Nematode)

Tags

EBFP

Expression

Worm

Promoter

Prig-3

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-OmpA-NHSTIIT-GG

Plasmid

#215790

Purpose

Cloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-SufI-NHSTIIT-GG

Plasmid

#215791

Purpose

Cloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-HIS6-SUMO1-AVITAG-GG

Plasmid

#215808

Purpose

Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MERS Mpro - His6-SUMO with autoprocessing - MSAVMQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL

Plasmid

#204790

Purpose

Bacterial expression of MERS Mpro

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS Mpro

Expression

Bacterial

Mutation

MSAVMQ/SGLVKM

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Sars Cov 2 Mac domain

Plasmid

#204787

Purpose

Bacterial expression of Sars Cov 2 Mac domain

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 Mac Domain (ORF1ab Sars Cov 2)

Expression

Bacterial

Available since

April 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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8TBX

Plasmid

#218228

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

DDX1 (DDX1 Human)

Use

Baculovirus expression

Tags

6xHis-TEV

Promoter

Polyhedrin

Available since

April 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-LASV-GPC-HA-c3xFLAG

Plasmid

#215691

Purpose

Produces the Lassa virus glycoprotein with a c-terminal HA tag and 3xFLAG tag

Depositor

Melinda Brindley

Publication

Willard et al Pathogens. 2018 Dec 26;8(1):1. doi: 10.3

Insert

LASV GP-HA-FLAG

Tags

HA-3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-LASV-GPC-c3xFLAG

Plasmid

#215689

Purpose

Produces the Lassa virus glycoprotein with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Publication

Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

Insert

LASV GP-FLAG

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_FBXW5:1-566

Plasmid

#210980

Purpose

Mammalian expression of full-length human FBXW5. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW5:M1-R566 (FBXW5 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_MAGEA3:104-314

Plasmid

#210941

Purpose

Insect expression of human MAGEA3 fragment E104 to E314. Untagged.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

MAGEA3:E104-E314 (MAGEA3 Human)

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-avi-TEV-LIC_DCAF12:1-453

Plasmid

#210940

Purpose

Insect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Expression

Insect

Mutation

R131Q natural variant (VAR_035322)

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_EIF2A:1-427

Plasmid

#210939

Purpose

Insect expression of human EIF2A fragment M1 to T427. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF2A:M1-T427 (EIF2A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_UTP15:1-370

Plasmid

#210937

Purpose

Insect expression of human UTP15 fragment M1 to H370. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

UTP15:M1-H370 (UTP15 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TBL2:70-405

Plasmid

#210936

Purpose

Insect expression of human TBL2 fragment E70 to H405. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TBL2:E70-H405 (TBL2 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SHKBP1:182-585

Plasmid

#210935

Purpose

Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SHKBP1:T182-L585 (SHKBP1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

Q507L natural variant (VAR_036714)

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_IFT172:1-320

Plasmid

#210934

Purpose

Insect expression of human IFT172 fragment M1 to S320. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

IFT172:M1-S320 (IFT172 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR19:1-636

Plasmid

#210933

Purpose

Insect expression of human WDR19 fragment M1 to G636. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR19:M1-G636 (WDR19 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR73:1-378

Plasmid

#210932

Purpose

Insect expression of full-length human WDR73. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR73:M1-R378 (WDR73 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TRAF7:382-670

Plasmid

#210930

Purpose

Insect expression of human TRAF7 fragment P382 to C670. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TRAF7:P382-C670 (TRAF7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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