Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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SARS-CoV-2 Mpro protease (M49T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204779

Purpose

Bacterial expression of Sars Cov 2 MPRO M49T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M49T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (M491I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204777

Purpose

Bacterial expression of Sars Cov 2 MPRO M49I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M49I

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (G143S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204776

Purpose

Bacterial expression of Sars Cov 2 MPRO G143S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

G143S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A191V mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204774

Purpose

Bacterial expression of Sars Cov 2 MPRO A191V mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A191V

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A193T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204773

Purpose

Bacterial expression of Sars Cov 2 MPRO A193T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A193T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A191T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204772

Purpose

Bacterial expression of Sars Cov 2 MPRO A191T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A191T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (A193S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204771

Purpose

Bacterial expression of Sars Cov 2 MPRO A193S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A193S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-SV40-Galpha-13 ONE-GO

Plasmid

#204332

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-13 ONE-GO (GNA13 Human)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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EV-A71 2A protease; 6xHis-tag, self cleaving

Plasmid

#204809

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Fusion of Dengue2 NS2B-NS3 proteins

Plasmid

#204793

Purpose

Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue2 NS2B-NS3 fusion

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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ZIKV NS2B-NS3 protease (H115A- catalytically inactive); aliases: Zika Virus NS2B-NS3 fusion protein expressed with N-terminal Tw

Plasmid

#204791

Purpose

Bacterial expression of a fusion of Zika NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika NS2B-NS3 fusion

Tags

6 His

Expression

Bacterial

Available Since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGL4.83(hRlucP/Puro)

Plasmid

#212934

Purpose

Renilla luciferase+PEST vector for investigating faster response to transcriptional changes

Depositor

Pete Stecha

Type

Empty backbone

Use

Luciferase

Mutation

WT

Available Since

Jan. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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WNV NS2B-NS3 protease: Co-expression of NS2B and NS3

Plasmid

#204806

Purpose

Bacterial co-expression of West Nile NS2B and NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B

Expression

Bacterial

Available Since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 2A protease; 6xHis-GST-TEV, low expression levels

Plasmid

#204804

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levels

Plasmid

#204803

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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WNV NS2B-NS3 protease (based on PDB 2IJO, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204799

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204797

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EV-D68 2A protease; no tag, low expression levels

Plasmid

#204805

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (R188K mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204770

Purpose

Bacterial expression of Sars Cov 2 MPRO R188K mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

R188K col1

Available Since

Nov. 30, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like

Plasmid

#204769

Purpose

Bacterial expression of Sars Cov 2 MPRO C145S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

C145S mutant

Available Since

Nov. 30, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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