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Showing: 1 - 30 of 9660 results

  1. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Aviv Regev
    Article
    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127546-plasmid-map-sequence-id-251803

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  2. pET28a MDHC_YEAST

    Plasmid
    #204232
    Purpose
    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHC_YEAST (MDH2 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204232-plasmid-map-sequence-id-413337

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  4. pET28a MDHM_YEAST

    Plasmid
    #204231
    Purpose
    Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM_YEAST (MDH1 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204231-plasmid-map-sequence-id-413335

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  5. pET28a MDHP_YEAST

    Plasmid
    #204233
    Purpose
    Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHP_YEAST (MDH3 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    The last 3 C-terminal residues (SKL) are the pero…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204233-plasmid-map-sequence-id-413332

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  9. p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

    Plasmid
    #52895
    Purpose
    Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Zipp.-L-IFP-F[1]
    Use
    Tags
    L-IFP-F[1]
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

    Plasmid
    #52893
    Purpose
    Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Zipp.-L-IFP-F[2]
    Use
    Tags
    L-IFP-F[2]
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

    Plasmid
    #52898
    Purpose
    YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Ypd*1-L-IFP-F[2]
    Use
    Tags
    L-IFP-F[2]
    Expression
    Yeast
    Mutation
    Changed Tryptophane 80 to Alanine
    Promoter
    GAL1
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pYB-Dual(mNG(yeast-opt))

    Plasmid
    #216727
    Purpose
    Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.
    Depositor
    Emil Jensen
    Article
    Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pGAL1(yeast) and pT7-RNAP(bacteria)
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    216727-plasmid-map-sequence-id-430980

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  19. pFA6a-link-ymScarletI3-URA3

    Plasmid
    #224075
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Use
    Tags
    ymScarletI3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224075-plasmid-map-sequence-id-460510

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  20. pFA6a-link-ymScarletI3-Kan

    Plasmid
    #224077
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Use
    Tags
    ymScarletI3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224077-plasmid-map-sequence-id-460512

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  21. pFA6a-link-ymiRFP680-URA3

    Plasmid
    #224102
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized miRFP680 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymiRFP680
    Use
    Tags
    ymiRFP680
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    224102-plasmid-map-sequence-id-453896

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  22. pFA6a-link-ymiRFP680-Kan

    Plasmid
    #224090
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized miRFP680 with Kan selection
    Depositor
    Bas Teusink
    Insert
    ymiRFP680
    Use
    Tags
    ymiRFP680
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224090-plasmid-map-sequence-id-452185

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  23. pFA6a-link-ymiRFP680-SpHis5

    Plasmid
    #224095
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized miRFP680 with SpHis5 selection
    Depositor
    Bas Teusink
    Insert
    ymiRFP680
    Use
    Tags
    ymiRFP680
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224095-plasmid-map-sequence-id-452412

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  24. pFA6a-link-yemiRFP670-URA3

    Plasmid
    #224100
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    yemiRFP670
    Use
    Tags
    yemiRFP670
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224100-plasmid-map-sequence-id-452374

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  25. pFA6a-link-ymScarletI3-SpHis5

    Plasmid
    #224076
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with SpHis5 selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Use
    Tags
    ymScarletI3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224076-plasmid-map-sequence-id-460513

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  26. pFA6a-link-yemiRFP670-Kan

    Plasmid
    #224088
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with Kan selection
    Depositor
    Bas Teusink
    Insert
    yemiRFP670
    Use
    Tags
    yemiRFP670
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224088-plasmid-map-sequence-id-452414

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  27. pFA6a-link-ymBaojin-URA3

    Plasmid
    #224081
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mBaojin with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymBaoJin
    Use
    Tags
    ymBaoJin
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224081-plasmid-map-sequence-id-452373

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  28. pFA6a-link-ymBaojin-SpHis5

    Plasmid
    #224082
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mBaojin with SpHis5 selection
    Depositor
    Bas Teusink
    Insert
    ymBaoJin
    Use
    Tags
    ymBaoJin
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224082-plasmid-map-sequence-id-452410

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  29. pFA6a-link-ymTurquoise2-Kan

    Plasmid
    #224092
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with Kan selection
    Depositor
    Bas Teusink
    Insert
    ymTurquoise2
    Use
    Tags
    ymTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224092-plasmid-map-sequence-id-453797

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  30. pFA6a-link-yemiRFP670-SpHis5

    Plasmid
    #224098
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with SpHis5 selection
    Depositor
    Bas Teusink
    Insert
    yemiRFP670
    Use
    Tags
    yemiRFP670
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224098-plasmid-map-sequence-id-453799

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Showing: 1 - 30 of 9660 results