Skip to main content
Addgene
Showing: 1 - 20 of 150 results

  1. pD649-HAsp-CD33-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156694
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD33 (CD33 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156694-plasmid-map-sequence-id-393026

    Enlarge

  3. Sdk1-AP-His

    Plasmid
    #72008
    Purpose
    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Sdk1 (Sdk1 Mouse)
    Use
    Tags
    AP-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72008-plasmid-map-sequence-id-135630

    Enlarge

  4. Sdk1-Fc-His

    Plasmid
    #72134
    Purpose
    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Sdk1 (Sdk1 Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72134-plasmid-map-sequence-id-139915

    Enlarge

  14. Beta1 Q280* K418* ectodomain

    Plasmid
    #221399
    Purpose
    Mammalian expression of human integrin beta1 Q280* K418* ectodomain
    Depositor
    Timothy Springer
    Article
    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.
    Insert
    integrin beta1 Q280* K418* ectodomain (ITGB1 Human)
    Use
    Tags
    AVI tag, HRV3C cleavage site, basic coil, HA tag,…
    Expression
    Mammalian
    Mutation
    codon optimized for human mature _1 residues Q1 t…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    221399-plasmid-map-sequence-id-439050

    Enlarge

  17. Beta1 N391* N562* ectodomain

    Plasmid
    #221400
    Purpose
    Mammalian expression of human integrin beta1 N391* N562* ectodomain
    Depositor
    Timothy Springer
    Article
    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.
    Insert
    integrin beta1 N391* N562* ectodomain (ITGB1 Human)
    Use
    Tags
    AVI tag, HRV3C cleavage site, basic coil, HA tag,…
    Expression
    Mammalian
    Mutation
    codon optimized for human mature _1 residues Q1 t…
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    221400-plasmid-map-sequence-id-437551

    Enlarge

  19. pD649-HAsp-SIGLEC6-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156762
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SIGLEC6 (SIGLEC6 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156762-plasmid-map-sequence-id-447056

    Enlarge

  20. R777-E089 Hs.FGFR4

    Plasmid
    #70373
    Purpose
    Gateway ORF clone of human FGFR4 [NM_002011.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    FGFR4 (FGFR4 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70373-plasmid-map-sequence-id-125822

    Enlarge

Showing: 1 - 20 of 150 results