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pETM33_TNKS_TANK-peptide-bind

Plasmid

#178489

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

TNKS_TANK-peptide-bind (TNKS Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pST39-Mega'-deltaNK

Plasmid

#216913

Purpose

Mega' with knockout of GvpN and GvpK

Depositor

George Lu

Insert

Mega' with knockout of GvpN and GvpK

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a human Tankyrase2 full length Y920E

Plasmid

#132639

Purpose

expresses human Tankyrase2 full length Y920E in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

Insert

Tankyrase2 Y920E

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse Tankyrase1 5ARC (171–961)

Plasmid

#132638

Purpose

expresses mouse Tankyrase1 5ARC (171–961) in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2018 Jun;27(6):1057-1067. d

Insert

Tankyrase1 (171–961)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)

Plasmid

#132637

Purpose

expresses mouse Tankyrase1 ARC2-3 (308-655) in E. coli

Depositor

Wenqing Xu

Article

Morrone et al Proc Natl Acad Sci U S A. 2012 Jan 31;10

Insert

Tankyrase1 (308-655)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMCV

Plasmid

#199743

Purpose

Minimal cloning vector

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.cc81-mut

Plasmid

#199745

Purpose

Expresses AAV2 Rep and AAV.cc81 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV.cc81 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

mutated amino acids 586-592 in Cap

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.cc84-mut

Plasmid

#199746

Purpose

Expresses AAV2 Rep and AAV.cc84 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV.cc84 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

mutated amino acids 586-592 in Cap

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-null

Plasmid

#200182

Purpose

Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV9 Cap w/ nonsense mutation at VR8

Use

AAV

Tags

Expression

Mammalian

Mutation

Replaced amino acids 586-592 in Cap with nonsense…

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pITR-AAV-Rep2Cap9-GFP

Plasmid

#199744

Purpose

Expresses AAV2 Rep and AAV9 Cap, with GFP replacing a region of Cap, within AAV2 ITRs.

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV9 Cap w/ GFP insert

Use

AAV

Tags

Expression

Mammalian

Mutation

Replaced amino acids 356-736 in Cap with GFP

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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CTraf

Plasmid

#27808

Depositor

Steven Vogel

Article

Thaler et al Biophys J. 2005 Oct . 89(4):2736-49.

Insert

Cerulean Traf (TANK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
pFLAG-TNKS-2

Plasmid

#34691

Depositor

Nai-Wen Chi

Article

Sbodio et al Biochem J. 2002 Feb 1;361(Pt 3):451-9.

Insert

TNKS2 (TNKS2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pFLAG-TNKS-2-M1054V

Plasmid

#34692

Depositor

Nai-Wen Chi

Article

Sbodio et al Biochem J. 2002 Feb 1;361(Pt 3):451-9.

Insert

TNKS2-M1054V (TNKS2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

M1054V

Promoter

CMV

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pGEMHE-TNKS-2

Plasmid

#154149

Purpose

For making TNKS-2 RNA

Depositor

Carmen Williams

Article

Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

Insert

Tankyrase 2 (TNKS2 Human)

Use

In vitro transcription

Tags

FLAG

Expression

Mutation

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-TNKS-2-M1054V

Plasmid

#138538

Purpose

For making TNKS-2-M1054V RNA

Depositor

Carmen Williams

Insert

Tankyrase 2 (TNKS2 Human)

Use

In vitro transcription

Tags

FLAG

Expression

Mutation

Changed methionine 1054 to valine

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-dusp6

Plasmid

#82584

Purpose

5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cells

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

FGF-responsive element of the dusp6 promoter

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-hPGK

Plasmid

#82579

Purpose

5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

Human PGK promoter

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pME-ERT2-Cre-ERT2

Plasmid

#82587

Purpose

Middle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombination

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

ERT2-CRE-ERT2

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-krt5

Plasmid

#82585

Purpose

5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

zebrafish krt5 promoter (krt5 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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