Addgene

We narrowed to 1,051 results for: SEF;

Showing: 361 - 400 of 1051 results

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pAAV-hSyn-JEDI-2P-Kv-WPRE

Plasmid

#179463

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV9

Insert

JEDI-2P-Kv

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-2P

Plasmid

#179458

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52379

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4

Use

Tags

Expression

Mammalian

Mutation

Promoter

OCT4

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPten#2/Cre

Plasmid

#173646

Purpose

Expresses a Pten-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Pten (Pten Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-GFAP-JEDI-2P-WPRE

Plasmid

#179469

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for astrocytes specific expression using the promoter GFAP

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/Puro-CAG-JEDI-2P-Kv

Plasmid

#179462

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P-Kv

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKIIa-JEDI-2P-WPRE

Plasmid

#179466

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-TARDBP-A315T

Plasmid

#141324

Purpose

Gateway cloning of TARDBP-A315T

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-A315T (TARDBP Human)

Use

Gateway cloning

Tags

Expression

Mutation

A315T

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKmt2d#1/Cre

Plasmid

#173597

Purpose

Expresses a Kmt2d-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Kmt2d (Kmt2d Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgKmt2d#2/Cre

Plasmid

#173598

Purpose

Expresses a Kmt2d-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Kmt2d (Kmt2d Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgTp53#2/Cre

Plasmid

#173621

Purpose

Expresses a Tp53-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Tp53 (Trp53 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgFbxw7#1/Cre

Plasmid

#173635

Purpose

Expresses a Fbxw7-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Fbxw7 (Fbxw7 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgArid1b#2/Cre

Plasmid

#173574

Purpose

Expresses a Arid1b-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Arid1b (Arid1b Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgArid1b#1/Cre

Plasmid

#173573

Purpose

Expresses a Arid1b-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Arid1b (Arid1b Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgArid2#1/Cre

Plasmid

#173575

Purpose

Expresses a Arid2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Arid2 (Arid2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgArid2#2/Cre

Plasmid

#173576

Purpose

Expresses a Arid2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Arid2 (Arid2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-TARDBP-G368A

Plasmid

#141325

Purpose

Gateway cloning of TARDBP-G368A

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-G368A (TARDBP Human)

Use

Gateway cloning

Tags

Expression

Mutation

G368A

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-TARDBP-W385G

Plasmid

#141326

Purpose

Gateway cloning of TARDBP-W385G

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-W385G (TARDBP Human)

Use

Gateway cloning

Tags

Expression

Mutation

W385G

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-TARDBP-G287A

Plasmid

#141323

Purpose

Gateway cloning of TARDBP-G287A

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-G287A (TARDBP Human)

Use

Gateway cloning

Tags

Expression

Mutation

G287A

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgCdkn2c#2/Cre

Plasmid

#173584

Purpose

Expresses a Cdkn2c-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Cdkn2c (Cdkn2c Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgMga#2/Cre

Plasmid

#173602

Purpose

Expresses a Mga-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Mga (Mga Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgAtrx#2/Cre

Plasmid

#173616

Purpose

Expresses a Atrx-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Atrx (Atrx Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgTp53#3/Cre

Plasmid

#173622

Purpose

Expresses a Tp53-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Tp53 (Trp53 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgAtrx#1/Cre

Plasmid

#173612

Purpose

Expresses a Atrx-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Atrx (Atrx Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgFbxw7#2/Cre

Plasmid

#173636

Purpose

Expresses a Fbxw7-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Fbxw7 (Fbxw7 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgPten#1/Cre

Plasmid

#173645

Purpose

Expresses a Pten-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Pten (Pten Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgCdkn2c#1/Cre

Plasmid

#173583

Purpose

Expresses a Cdkn2c-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Cdkn2c (Cdkn2c Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgArhgap35#2/Cre

Plasmid

#173570

Purpose

Expresses a Arhgap35-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Arhgap35 (Grlf1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgArhgap35#1/Cre

Plasmid

#173569

Purpose

Expresses a Arhgap35-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Arhgap35 (Grlf1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-human OCT4 PE-SV40-Luc

Plasmid

#52415

Purpose

Luciferase reporter plasmid for PE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Proximal element of hOCT4 promoter (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLD-puro-Cc-TARDBP-WT_VA

Plasmid

#141327

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-WT (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

Plasmid

#52419

Purpose

Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

ERAS (ERAS Human)

Use

Tags

Expression

Mutation

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE

Plasmid

#179459

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV1

Insert

JEDI-2P-Kv

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-human OCT4 DE-SV40-Luc

Plasmid

#52414

Purpose

Luciferase reporter plasmid for DE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Distal element of human OCT4 promoter (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
delta PE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52380

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -1620 - -1011 upstream from OCT4 ATG

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLD-puro-Cc-TARDBP-A315T_VA

Plasmid

#141329

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-A315T in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-A315T (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Mutation

A315T

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
delta DE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52381

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -3194 - -1768 upstream from OCT4 ATG

Promoter

OCT4

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mouse deltaPE Oct4-GFP transgenic reporter

Plasmid

#52382

Purpose

BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP pA
Oct4 (Pou5f1 Mouse)
PGK-gb2-Neo

Use

Tags

Expression

Mammalian

Mutation

deleted -300 - -1300bp fragment upstream from Oct…

Promoter

Oct4 and PGK+gb2

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLD-puro-Cc-TARDBP-G368A_VA

Plasmid

#141330

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-G368A in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-G368A (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Mutation

G368A

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLD-puro-Cc-TARDBP-G287A_VA

Plasmid

#141328

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-G287A in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-G287A (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Mutation

G287A

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 361 - 400 of 1051 results

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