Addgene

Showing: 181 - 200 of 403 results

Results per page:

Sort results by:

JAB2518

Plasmid

#190384

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-NLS_19St)

Depositor

Jose Dinneny, Jennifer Brophy

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::RpaR-NLS_19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2494

Plasmid

#190376

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-VP16_19St)

Depositor

Jose Dinneny, Jennifer Brophy

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::RpaR-VP16_19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
KFP14

Plasmid

#119796

Purpose

RpoD6 epitope tagged construct for integration at neutral site 1

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1557 (Synpcc7942_1557 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KFP13

Plasmid

#119795

Purpose

RpoD5 epitope tagged construct for integration at neutral site 1

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1849 (Synpcc7942_1849 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KFP06

Plasmid

#119799

Purpose

RpoD6 epitope tagged construct for integration at neutral site 2.2

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1557 (Synpcc7942_1557 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
KFP05

Plasmid

#119800

Purpose

RpoD5 epitope tagged construct for integration at neutral site 2.2

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1849 (Synpcc7942_1849 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-6h-GST-TEVp

Plasmid

#172887

Purpose

Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Insert

TEV protease

Use

Tags

GST solubility tag and HHHHHH

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSecEffector

Plasmid

#172891

Purpose

Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Inserts

Bxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites

Use

Tags

Expression

Bacterial

Mutation

Promoter

ProD, nTETO, and pBAD /AraC

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2511

Plasmid

#190380

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XRpaRO-min35S::GFPint-ADHt)

Depositor

Jose Dinneny, Jennifer Brophy

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XRpaRO-min35S::GFPint-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFOSGE_COF1E01

Plasmid

#70777

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

RpLP2 (RpLP2 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
lentiCRISPR-v2-HygR-mCherry

Plasmid

#167189

Purpose

Lentiviral expression of sgRNA with hygromycin resistance gene and mCherry

Depositor

Yin Shen

Article

Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

Insert

Hygromycin B phosphotransferase and mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-PelB-6H-Ag43-160N-sfGFP

Plasmid

#172886

Purpose

Display sfGFP protein on E. coli cell surface

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Inserts

SfGFP
Ag43, partial (flu )

Use

Tags

HHHHHH and TEV recognition site (ENLYFQG)

Expression

Bacterial

Mutation

Deleted 1-160 (N terminal)

Promoter

T7 Promoter

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pH6HTC_HNRNPA1-Halo

Plasmid

#175307

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HNRNPA1 (HNRNPA1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR-v2-Blast-Puro

Plasmid

#167186

Purpose

Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes

Depositor

Yin Shen

Article

Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

Insert

Blasticidin S deaminase

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p156 pWZL-EGFP-SLPI

Plasmid

#11069

Depositor

Ramesh Shivdasani

Article

Schulze et al Blood. 2004 Dec 15. 104(13):3949-57.

Insert

SLPI (Slpi Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
lentiCRISPR-v2-HygR-EGFP

Plasmid

#167188

Purpose

Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

Depositor

Yin Shen

Article

Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

Insert

Hygromycin B phosphotransferase and EGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-hApg5

Plasmid

#22952

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-LC3-YFP

Plasmid

#99571

Purpose

Lentiviral expression of YFP-tagged LC3

Depositor

Edward Campbell

Article

Flavin et al Acta Neuropathol. 2017 May 19. doi: 10.1

Insert

LC3 (MAP1LC3B Human)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-hApg5-HA

Plasmid

#22948

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pBR322-MCK1.35lux

Plasmid

#53368

Purpose

expresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancer

Depositor

Josephine Nalbantoglu

Article

Larochelle et al Gene Ther. 1997 May;4(5):465-72.

Insert

muscle creatine kinase promoter/enhancer 1.35kb

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only