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We narrowed to 1,094 results for: gibson assembly

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Showing: 991 - 1000 of 1094 results

  1. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  2. p2R3z-BASI-ccdB-BSAI

    Plasmid
    #118389
    Purpose
    Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4
    Insert
    ccdB
    Use
    Unspecified
    Mutation
    See Depositor Comments Section
    Available Since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    118389-plasmid-map-sequence-id-470418

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  4. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181784-plasmid-map-sequence-id-358224

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  5. pNB-Rep

    Plasmid
    #238996
    Purpose
    Node B carrying the sfGPF reporter that can be used for the assembly of pEND-11Y, pEND-Y11, pEND-Z11 or pEND-ZY
    Depositor
    Javier Santos-Moreno
    Article
    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2
    Insert
    sfGFP-MarAn10 downstream of a strong constitutive promoter and a binding site for sgRNA-1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    238996-plasmid-map-sequence-id-495027

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Showing: 991 - 1000 of 1094 results