2,226 results

1. pGEX-4T Syt1 C2AB(140) I367C

   Plasmid

   #134699

   Purpose

   Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Synaptotagmin 1 (Syt1 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   I367C, C277A

   Promoter

   Available since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIVT-EGFP-ratCENP-C

   Plasmid

   #174445

   Purpose

   in vitro transcription template for rat CENP-C (N-ter EGFP tagged)

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Centromere protein C
   

   Use

   In vitro transcription template

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   T7 SP6

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP

   Plasmid

   #135563

   Purpose

   An AAV vector expressing scrambled shRNA and a nuclear EYFP reporter

   Depositor

   Christopher Richie

   Article

   Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

   Inserts

   shRNA (scrambled)
   Nuc-EYFP
   

   Use

   AAV and RNAi

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE and mU6

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLIC-His-GBAi

   Plasmid

   #171755

   Purpose

   For the expression of His-GBAi in bacteria

   Depositor

   Mikel Garcia-Marcos

   Article

   Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

   Insert

   GBAi
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

   Plasmid

   #167478

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(I539E)-ShadowG
   

   Use

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

   Plasmid

   #167479

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(C450A)-ShadowG
   

   Use

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETM10 with rat Δ192Annexin A11

   Plasmid

   #164498

   Purpose

   For bacterial expression of rat His-tagged Δ192Annexin A11

   Depositor

   Anni Vedeler

   Article

   Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

   Insert

   rat Δ192Annexin A11 (Anxa11 Rat)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   N-terminal truncation

   Promoter

   T7

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETM10 with rat Δ188Annexin A11

   Plasmid

   #164497

   Purpose

   For bacterial expression of rat His-tagged Δ188Annexin A11

   Depositor

   Anni Vedeler

   Article

   Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

   Insert

   rat Δ188Annexin A11 (Anxa11 Rat)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   N-terminal truncation

   Promoter

   T7

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Ad-HisFlag-USP19ER

   Plasmid

   #155244

   Purpose

   for generating adenoviruses expressing the ER isoform of rat USP19

   Depositor

   Simon Wing

   Article

   Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

   Insert

   USP19 (ER isoform) (Usp19 Rat)
   

   Use

   Adenoviral

   Tags

   6XHis-3XFlag

   Expression

   Mutation

   H118R- please see dep. comments

   Promoter

   CMV

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCIS-rPAM3

    Plasmid

    #145803

    Purpose

    expresses rat PAM3 in mammalian cells

    Depositor

    Richard Mains, Betty Eipper

    Article

    Milgram et al J Cell Biol. 1994 Jan;124(1-2):33-41. do

    Insert

    rat PAM3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-rUSP19ER-siRNAresistant

    Plasmid

    #155251

    Purpose

    for mammalian overexpression of the siRNA resistant ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (ER isoform) siRNA resistant (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

    Promoter

    CMV

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-rUSP19CYT-siRNAresistant

    Plasmid

    #155252

    Purpose

    for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

    Promoter

    CMV

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-HisFlag-rUSP19ER-CAmut

    Plasmid

    #155237

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-HisFlag-rUSP19CSdomains

    Plasmid

    #155240

    Purpose

    for mammalian overexpression of the CS domains of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (CS domains) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CBSH3+ shRNA-2

    Plasmid

    #160068

    Purpose

    Knock Down SH3+ Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CBSH3+ 3m shRNA-1

    Plasmid

    #160067

    Purpose

    3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    GGcTGaTTaCAACAAGGATTG (mutations shown in lower c…

    Promoter

    mouse U6 promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CBSH3+ shRNA-1

    Plasmid

    #160066

    Purpose

    Knock Down SH3+ Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CBSH3- 3m shRNA3

    Plasmid

    #160213

    Purpose

    Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    TGTATGACCTCaGGcTGtACCA (mutations shown in lower …

    Promoter

    mouse U6 promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CBSH3- shRNA2

    Plasmid

    #160210

    Purpose

    Knock Down MTLSH3- Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat, Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CBSH3- shRNA3

    Plasmid

    #160212

    Purpose

    Knock Down MYDSH3- Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat, Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge