2,294 results

1. pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

   Plasmid

   #198844

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   COX2-Q6-L18 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3-MTS-ND2-W30-L16-G1333C-UGI-Puro

   Plasmid

   #198852

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ND2-W30-L16 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro

   Plasmid

   #198848

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   CYTB-W77-L15 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro

   Plasmid

   #198842

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   COX1-W25-L15 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLIK mCh-V5-NES-EGFP-Erbb2 NLS zeo

   Plasmid

   #192339

   Purpose

   Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN_TT mCh-V5-NES-EGFP-Erbb2 NLS

   Plasmid

   #192317

   Purpose

   Gateway entry vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe NGF-Erbb2-2xFKBP-HA hygro

   Plasmid

   #192291

   Purpose

   Retroviral expression vector for NGF-Erbb2-2xFKBP-HA construct

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   NGF-Erbb2-2xFKBP-HA (Erbb2 Rat)
   

   Use

   Retroviral

   Tags

   HA

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro

   Plasmid

   #198860

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ND6-W82-L15 TALEN
   

   Expression

   Mammalian

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP

   Plasmid

   #194016

   Purpose

   Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

   Depositor

   Larry Young

   Article

   Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

   Inserts

   sgRNA(Oxtr.2)
   eGFP
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   cmb and u6

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCI-neo-μ3A-(HA)3

    Plasmid

    #198179

    Purpose

    Expression of HA-tagged AP-3 μ3A in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

    Insert

    AP-3 μ3A
    

    Tags

    triple HA tag

    Expression

    Mammalian

    Mutation

    silent substitution in codon 225

    Promoter

    CMV

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. B52_puro_gRNA_3418711_KCNB2

    Plasmid

    #197558

    Purpose

    gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)

    Depositor

    Darius Bägli

    Insert

    upstream KCNB2 promoter gRNA (Kcnb2 Rat)
    

    Use

    Grna with puromycin selection

    Tags

    puromycin resistance cassette under CMV promotion

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RCav3.1/pcDNA3-HE3

    Plasmid

    #197324

    Purpose

    Plasmid expressing an antigen targeting the Cav3.1 calcium channel subunit

    Depositor

    Edward Perez-Reyes

    Insert

    Cav3.1 (Cacna1g Rat)
    

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GIT1-Flag/pBK

    Plasmid

    #197313

    Purpose

    Plasmid expressing an antigen targeting GIT1

    Depositor

    Eunjoon Kim

    Insert

    GIT1 (Git1 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGADT7-AP-2 σ2

    Plasmid

    #198345

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-2 σ2
    

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    R42G substitution, and silent substitutions in co…

    Promoter

    ADH1

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPOZ0CM0137

    Plasmid

    #197535

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Article

    Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

    Insert

    C-terminal glucocorticoid receptor, GR
    

    Use

    Synthetic Biology

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMeCP2G pc1121

    Plasmid

    #167566

    Purpose

    The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

    Depositor

    Cristina Cardoso

    Article

    Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

    Inserts

    Methyl-CpG binding protein 2 (Mecp2 Rat)
    GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMeCP2miRFP670 pc3942

    Plasmid

    #167568

    Purpose

    The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

    Depositor

    Cristina Cardoso

    Article

    Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

    Insert

    MeCp2 (Mecp2 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMeCP2Y pc644

    Plasmid

    #167565

    Purpose

    The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)

    Depositor

    Cristina Cardoso

    Article

    Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

    Insert

    Methyl-CpG binding protein 2 (Mecp2 Rat)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMyosin-miRFP718nano

    Plasmid

    #195748

    Purpose

    LC-Myosin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-LC-Myosin
    

    Tags

    miRFP718nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PH-miRFP-FRBx2

    Plasmid

    #193569

    Purpose

    Expresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    PH domain from PLC delta1 (Plcd1 Rat)
    

    Tags

    FRB (T2098L) and miRFP703

    Expression

    Mammalian

    Mutation

    PH domain

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge