Addgene

We narrowed to 3,518 results for: Mak

Showing: 2971 - 3000 of 3518 results

Results per page:

Sort results by:

pTa-HH5-1

Plasmid

#124995

Purpose

To make transgenic mice for HH5-1 TCRa

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1 TCRa Tg

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-ceRab2(1-214)

Plasmid

#59550

Purpose

makes 6x Fusion of Full length ceRab2

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-214

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab5(1-208)

Plasmid

#59551

Purpose

makes 6x Fusion of Full length ceRab5

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-208

Use

Tags

6x His

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab10(1-201)

Plasmid

#59552

Purpose

makes 6x Fusion of Full length ceRab10

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-208

Use

Tags

6x His

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab11(1-211)

Plasmid

#59553

Purpose

makes 6x Fusion of Full length ceRab11

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-211

Use

Tags

6x His

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab21(1-207)

Plasmid

#59554

Purpose

makes 6x Fusion of Full length ceRab21

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-207

Use

Tags

6x His

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab21(1-209)

Plasmid

#59555

Purpose

makes 6x Fusion of Full length ceRab35

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-209

Use

Tags

6x His

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
bactin2:loxP-STOP-loxP-DsRed-express

Plasmid

#68797

Purpose

For making transgenic fish

Depositor

David Traver

Article

Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

Inserts

beta-actin promoter
loxp-stop-codon-loxp-dsred

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-mGarnet2

Plasmid

#104312

Purpose

codes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cells

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

H2B-mGArnet2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSSM110: pCMV-HA-NLS-SpuFz1-v2

Plasmid

#205277

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

C310R/D487K/T513K

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ134B2.0

Plasmid

#167158

Purpose

Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU3

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
modRNAc1-VP64dCas9VP64-2A-MPH

Plasmid

#216388

Purpose

this vector can be used to make modRNA expressing VP64dCas9VP64 and MPH

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

VP64dCas9VP64-2A-MPH

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA5c-MevT-MBI (JBEI-3085)

Plasmid

#87950

Purpose

the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

MevT-MBI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TREtight-mTagBFP2-N2cG

Plasmid

#192838

Purpose

plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

Depositor

Ian Wickersham

Insert

mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TREtight

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCSGC-GB1

Plasmid

#186786

Purpose

E. coli expression vector containing N-terminal 6His-GB1-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ135B2.0

Plasmid

#167159

Purpose

Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU3

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
modRNAc1-Cas9-2A-p53DD

Plasmid

#216389

Purpose

this vector can be used to make modRNA expressing Cas9 and p53DD

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

Cas9-2A-p53DD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJET1.2-STOP-dsRed

Plasmid

#60944

Purpose

Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

dsRED

Use

Unspecified

Tags

Expression

Mutation

Promoter

3XP3

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ136B2.0

Plasmid

#167160

Purpose

Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU3

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p221z-CAS9p-t35s

Plasmid

#118385

Purpose

Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSSM16: pCMV-HA-NLS-SpuFz1

Plasmid

#205271

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p221z-dCAS9p-t35s

Plasmid

#118387

Purpose

Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

dCAS9p

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
modRNAc1-Cas9-2A-puro

Plasmid

#216390

Purpose

this vector can be used to make modRNA expressing Cas9 and puro resistance gene

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

Cas9-2A-puro

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Right-stuffer-1397N

Plasmid

#187172

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397N

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDZ585 pKAN 2x-mCherry

Plasmid

#72236

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

2x-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pERB221

Plasmid

#61502

Purpose

const. CenpB-GFP-Halo + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

CenpB-GFP-Halo
mCherry-DHFR

Use

Tags

HaloTag and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-Lifeact-mEos2-NS

Plasmid

#115549

Purpose

Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Lifeact-mEos2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pERB217

Plasmid

#61500

Purpose

Const. Halotag-GFP-Mito + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-Mito
mCherry-DHFR

Use

Tags

HaloTag and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLC242-GFP-Sestrin2

Plasmid

#100519

Purpose

Expresses GFP tagged Sestrin2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SESN2 (SESN2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pERB215

Plasmid

#61499

Purpose

Const. Halotag-GFP-Nuf2 + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-Nuf2
mCherry-DHFR

Use

Tags

HaloTag and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge