We narrowed to 4,145 results for: pes

1. pAAV-CaMKII-VARNAM2-Kv2.1PR

   Plasmid

   #195530

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   CaMKII

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBV65

   Plasmid

   #108363

   Purpose

   A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

   Depositor

   Scott Moye-Rowley

   Article

   Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

   Insert

   loxP-natMX6-CgMET3prom-cre-loxP
   

   Use

   Gene disruption marker for candida glabrata

   Tags

   Expression

   Mutation

   Promoter

   C. glabrata MET3

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fireworks GFP[PTC-] (AVA2598)

   Plasmid

   #85445

   Purpose

   Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1_GI.1_WT

   Plasmid

   #162579

   Purpose

   Expresses norovirus GI.1 VP1 protein in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78304

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADfluw-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nrxn3α-AS4-

   Plasmid

   #184332

   Purpose

   Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

   Depositor

   Peter Scheiffele

   Article

   Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

   Insert

   Nrxn3-alpha (Nrxn3 Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH2R-mCherry-N1

   Plasmid

   #84326

   Purpose

   Histamine-2 receptor tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   H2R (HRH2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

   Plasmid

   #121976

   Purpose

   transfection studies in eukaryotic cells such as COS-7 cells

   Depositor

   Christophe Erneux

   Article

   De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

   Insert

   Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAD16_CAD_12-490_pvp008

   Plasmid

   #124842

   Purpose

   Expresses Aspergillus terreus CAD in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-11, synthetic codon optimiz…

   Promoter

   T7

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Syn-pAceR

    Plasmid

    #195521

    Purpose

    Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    Synapsin

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FDIO-COX4-dAPEX2

    Plasmid

    #117178

    Purpose

    Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    COX4-dAPEX2
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nrxn3α-AS4+

    Plasmid

    #184333

    Purpose

    Expression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

    Depositor

    Peter Scheiffele

    Article

    Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

    Insert

    Nrxn3-alpha (Nrxn3 Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-Regnase-1 WT

    Plasmid

    #222653

    Purpose

    Mammalian expression vector to express mouse Regnase-1 WT

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a (Zc3h12a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-FluoSTEP-JNKAR

    Plasmid

    #181857

    Purpose

    Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-JNKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid

    #181856

    Purpose

    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AktAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

    Plasmid

    #120269

    Purpose

    Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

    Depositor

    Edward Callaway

    Article

    Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

    Insert

    eSyn-DIO-TVA950:YFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas4_1_2_VA

    Plasmid

    #186438

    Purpose

    Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWPT-mCherry

    Plasmid

    #190605

    Purpose

    Obtained by cloning 5 stop codons in place of the EGFP Kozak sequence in pWPT-/mEGFP-1T-IRES-mCherry

    Depositor

    Alessandro Quattrone

    Article

    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP Kozak sequence has been replaced with a stre…

    Promoter

    EIF1-short

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

    Plasmid

    #181859

    Purpose

    Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    cp-PKN-mRuby2-GFP(1-0)
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-FDIO-LACTB-dAPEX2

    Plasmid

    #117181

    Purpose

    Plasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    LACTB-dAPEX2
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge