165,750 results

1. dCas9-10xSunTag-P2A-tagBFP

   Plasmid

   #174141

   Purpose

   Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, 10xSunTag, tagBFP
   

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1(nsp8-nsp7)(nsp12)

   Plasmid

   #165451

   Purpose

   Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

   Depositor

   Marc Delarue

   Article

   Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

   Inserts

   nsp12
   nsp8
   nsp7
   

   Tags

   14Histidine-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

   Plasmid

   #192664

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR CD19-mMegf10 CAR

   Plasmid

   #113013

   Purpose

   for production of lentivirus encoding the CD19 CAR-Pmegf10

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   CD19 CAR-P Megf10 intracellular domain (CD19, Megf10 Synthetic, Mouse, Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-mSA-EGFP-TM

   Plasmid

   #39863

   Depositor

   Sheldon Park

   Article

   Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

   Insert

   monomeric streptavidin, EGFP, TM helix
   

   Tags

   EGFP, FLAG, and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-Merlin S518A

   Plasmid

   #84294

   Purpose

   Expression of Merlin with an alanine mutation at the Pak phosphorylation site

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Merlin (NF2) (NF2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S518A

   Promoter

   CMV

   Available Since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-TRE-DIO-hM3Dq-mCherry

   Plasmid

   #166599

   Purpose

   Encodes Cre-dependent hM3Dq-mCherry under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Promoter

   tetracycline response element

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG416GAL-ccdB

   Plasmid

   #14147

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Gateway destination

   Expression

   Yeast

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-MbPylRS(DiZPK)

   Plasmid

   #91706

   Purpose

   express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

   Depositor

   P. Chen

   Article

   Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

   Inserts

   pylS
   pylT
   

   Expression

   Mammalian

   Mutation

   T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPLICSFAST-LY-MT-P2A

    Plasmid

    #214897

    Purpose

    Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-LY-MT-P2A
    

    Tags

    none

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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