We narrowed to 10,632 results for: t7

1. 2OQK

   Plasmid

   #60112

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Cp-PF11_0447:MAC01Z-A10:C31333
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   Nov. 19, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. 2P65

   Plasmid

   #60113

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Pv089580:MAC01Q-D10:C26843
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. 4JWQ

   Plasmid

   #60084

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PB000947.00.0:MAC03Z-H05:C218239
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. 4LDF

   Plasmid

   #60089

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   cgd3_3190:MAC04H-E05:C228722
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. 4LDG

   Plasmid

   #60090

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   cgd4_370:MAC04H-G03:C228753
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. JMC01B-H12

   Plasmid

   #58758

   Purpose

   bacterial expression of PDB 4O62

   Depositor

   Cheryl Arrowsmith

   Insert

   PDB:4O62 (ZCWPW2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   Aug. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pRSET iGluSnFR3 v857

   Plasmid

   #175186

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   iGluSnFR3 v857
   

   Use

   Synthetic Biology

   Tags

   His tag, T7 leader, Xpress epitope

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPos-Ma-CmR-TAG111

   Plasmid

   #197571

   Purpose

   Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   Chloramphenicol resistance gene - 111TAG
   T7 polymerase -TAG
   GFPuv
   M. alvus Pyl-tRNA(6)
   

   Expression

   Bacterial

   Mutation

   111TAG and 8TAG/112TAG

   Promoter

   AraC, T7, cat promoter (constitutive), and lpp (c…

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGGS-PHFF-sh-hBVRA

   Plasmid

   #100285

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Inserts

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
   shRNA for human BVRA
   

   Tags

   FLAG, T7 and Mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   All genes are codon-optimized for expression in h…

   Promoter

   CAG promoter and H1 promoter

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSET iGluSnFR3 v82

    Plasmid

    #175187

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    iGluSnFR3 v82
    

    Use

    Synthetic Biology

    Tags

    His tag, T7 leader, Xpress epitope

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ATX3-77Q

    Plasmid

    #184249

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Human, Synthetic)
    

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Codon optimization for protein expression in BL2…

    Promoter

    T7 promoter

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-PHFF-sh-mBVRA

    Plasmid

    #100286

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-Kss1

    Plasmid

    #52682

    Purpose

    Expression of Kss1 in bacteria

    Depositor

    Benjamin Turk

    Article

    Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

    Insert

    KSS1 (KSS1 Budding Yeast)
    

    Tags

    His6 and T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

14. ATX3-77Q-R388G

    Plasmid

    #184251

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Human, Synthetic)
    

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    C-terminal codon optimization for protein expres…

    Promoter

    T7 promoter

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ABL1-C-HiBiT

    Plasmid

    #234568

    Purpose

    Bacterially expressed ABL1-C-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    Abelson Kinase Domain (ABL1 Human)
    

    Tags

    HiBiT Tag-thrombin site-10xHis and T7 gene 10 lea…

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    T7

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEMHE-RnCENP-O-11NS-GFP

    Plasmid

    #205181

    Purpose

    Expresses rat CENP-O with 11 mouse-specific point mutations in residues under positive selection; C-term tagged with GFP under T7 promoter - designed for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

    Insert

    CENP-O (Cenpo, Cenpo Rat, Mouse)
    

    Use

    In vitro transcription

    Tags

    5 glycines linker followed by GFP

    Expression

    Mammalian

    Mutation

    Rat CENP-O with 11 mouse-specific point mutations…

    Promoter

    T7

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEMHE-RnCENP-O-203-GFP

    Plasmid

    #205183

    Purpose

    Expresses chimeric rat CENP-O with mouse CENP-O middle region including central RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

    Insert

    CENP-O (Cenpo, Cenpo Rat, Mouse)
    

    Use

    In vitro transcription

    Tags

    5 glycines linker followed by GFP

    Expression

    Mammalian

    Mutation

    Chimeric rat CENP-O with mouse CENP-O middle regi…

    Promoter

    T7

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEMHE-RnCENP-O-298-GFP

    Plasmid

    #205184

    Purpose

    Expresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

    Insert

    CENP-O (Cenpo, Cenpo Rat, Mouse)
    

    Use

    In vitro transcription

    Tags

    5 glycines linker followed by GFP

    Expression

    Mammalian

    Mutation

    Chimeric rat CENP-O with mouse CENP-O C-terminal …

    Promoter

    T7

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEMHE-MmCENP-O-R298-GFP

    Plasmid

    #205185

    Purpose

    Expresses chimeric mouse CENP-O with rat CENP-O C-terminal RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

    Insert

    CENP-O (Cenpo, Cenpo Rat, Mouse)
    

    Use

    In vitro transcription

    Tags

    5 glycines linker followed by GFP

    Expression

    Mammalian

    Mutation

    Chimeric mouse CENP-O with rat CENP-O C-terminal …

    Promoter

    T7

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMBP-HsSyx3

    Plasmid

    #179287

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Tags

    His6 + mature maltose binding protein + TEV prote…

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge