159,557 results

1. pET21-pelB-LaMIgG-14

   Plasmid

   #228994

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-14
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaTdT-39

   Plasmid

   #228980

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-39
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MZB134

   Plasmid

   #228485

   Purpose

   "Empty" N-terminal 6xHis-TEV-Halotag-HRV vector for easy DNA Assembly for pulldowns (Bacterial Expression)

   Depositor

   Richard Baker

   Type

   Empty backbone

   Use

   Tags

   6xHis-TEV-Halo-HRV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOGS539_haPD-1

   Plasmid

   #226716

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   High affinity PD-1 microbody
   

   Use

   Tags

   HA tag and Mating factor alpha secretion signal

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOGS539_rPD-L1nb

   Plasmid

   #226717

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Recombinant PD-L1 nanobody
   

   Use

   Tags

   HA tag, Histidine tag, and Mating factor alpha se…

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTBY1-T4uvsY

   Plasmid

   #228214

   Purpose

   Expression in BL21(DE3) of T4 UvsY-like recombination mediator

   Depositor

   Stephen Benkovic

   Insert

   uvsY (uvsY Escherichia phage T4)
   

   Use

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-SnoopTag-AffiHER2-SpyTag

   Plasmid

   #216280

   Purpose

   Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SnoopTag-AffiHER2-SpyTag
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBOBI TP53 S46D

   Plasmid

   #213002

   Purpose

   expresses human P53

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   P53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S46D

   Promoter

   cmv

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBiT3.1-C_FZR1:1-496

   Plasmid

   #211170

   Purpose

   Mammalian expression of full-length human FZR1. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   FZR1:M1-R496 (FZR1 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4_CRE-CMV-BC0502-luc2

    Plasmid

    #214644

    Purpose

    barcoded assay, cAMP/Ca2+ sensor

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    6x clustered CRE element with barcode 0502
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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