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pCMV-PE2-P2A-GFP

Plasmid

#132776

Purpose

Prime editing in mammalian cells with co-translational GFP expression

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2-P2A-GFP

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLTR-RD114A

Plasmid

#17576

Depositor

Jakob Reiser

Article

Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Insert

RD114 envelope glycoprotein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Industry, Academic Institutions, and Nonprofits

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ABE8e

Plasmid

#138489

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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dCAS9-VP64_GFP

Plasmid

#61422

Purpose

Expresses dCAS9-VP64 activator with 2A GFP

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Concentrated Lentiviral Prep

Insert

dCAS9(D10A,H840A)-VP64_2A_GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

EF1A

Available since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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PX552

Plasmid

#60958

Purpose

pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

Depositor

Feng Zhang

Article

Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

Inserts

U6_(SpaI)_sgRNA
Syn_EGFP-KASH

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin and U6

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG2(11)1-10

Plasmid

#82610

Purpose

Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

SFFV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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FRESCA-syntide

Plasmid

#132415

Purpose

FRET-based sensor for CaMKII activity

Depositor

Margaret Stratton

Article

Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

Insert

FRESCA-syntide

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#69496

Purpose

pJL1 expression vector with sfGFP

Depositor

Michael Jewett

Article

Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

Insert

Super folder GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-M-puro

Plasmid

#125839

Purpose

modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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