We narrowed to 31,888 results for: Eng

1. (B) BLE marker(R) (SBE262)

   Plasmid

   #195012

   Purpose

   bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   pXYL+BLE+tGPD
   

   Expression

   Bacterial

   Promoter

   pXYL

   Available Since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZ_lacO-cP-AOX1-eGFP

   Plasmid

   #126720

   Purpose

   yeast plasmid overexpressing eGFP

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   egfp
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p1585588

   Plasmid

   #62620

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   dihydroflavonol 4-reductase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   leucoanthocyanidin reductase
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCHC025 (∆CBBp)

   Plasmid

   #226203

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
   

   Use

   Synthetic Biology

   Mutation

   ∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpAp

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCHC024 (∆SH)

   Plasmid

   #226202

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for soluble hydrogenase operon
   

   Use

   Synthetic Biology

   Mutation

   ∆hoxFUYHWI ∆hypA2B2F2

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCHC023 (∆MBH)

   Plasmid

   #226201

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for membrane bound hydrogenase operon
   

   Use

   Synthetic Biology

   Mutation

   ∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCHC027 (∆pemK)

   Plasmid

   #226204

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Upstream/downstream homology arms for addiction system toxinPemK.
   

   Use

   Synthetic Biology

   Available Since

   Nov. 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pACB108

   Plasmid

   #222207

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACB109

   Plasmid

   #222208

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA (K10N)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Lys10 to Asn

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACB110

    Plasmid

    #222209

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA (G258D)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Changed Gly258 to Aspartate

    Promoter

    T7

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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