2,226 results

1. RnAT1aR DRY->AAY-mCherry

   Plasmid

   #137783

   Purpose

   Visualization of the At1aR DRY mutant

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat At1a receptor; DRY mutant (Agtr1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DRY -> AAY

   Promoter

   CMV

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK3b_015

   Plasmid

   #123796

   Purpose

   Encodes rat Connexin 26 as a Type 3b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Cx 26 (rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK3b_017

   Plasmid

   #123798

   Purpose

   Encodes rat Connexin 43 as a Type 3b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Cx43 (rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MBP-rPKL S12TAG

   Plasmid

   #107155

   Purpose

   MBP-rPKL construct for production and purification of rat PKL pS12

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
   

   Use

   Tags

   C-term 6-his and N-term MBP

   Expression

   Bacterial

   Mutation

   Serine 12 to TAG for phosphoserine insertion

   Promoter

   PBAD

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MBP-rPKL S113TAG

   Plasmid

   #107157

   Purpose

   MBP-rPKL S113TAG for production and purification of rat PKL pS113

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
   

   Use

   Tags

   C-term 6-his and N-term MBP

   Expression

   Bacterial

   Mutation

   Serine 113 changed to TAG for phosphoserine inser…

   Promoter

   PBAD

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. prNFL-P22S-EGFP

   Plasmid

   #132594

   Purpose

   Expresses hEGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P22S mutation

   Depositor

   Anthony Brown

   Article

   Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

   Insert

   NEFL (Nefl Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   64C>A, g5C>G, Pro22Ser

   Promoter

   CMV

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPRIME-CMV-GFP-shNts2

   Plasmid

   #132714

   Purpose

   Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shNts(2) (Nts Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPRIME-CMV-GFP-shNts1

   Plasmid

   #132713

   Purpose

   Encodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts gene

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shNts(1) (Nts Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPRIME-CMV-GFP-shCrh2

   Plasmid

   #132710

   Purpose

   Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh gene

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shCrh(2) (Crh Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2+ rat MYO1D

    Plasmid

    #118419

    Purpose

    For mammalian expression of rat myosin 1D

    Depositor

    Michael Tsang

    Article

    Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

    Insert

    rat myosin 1D (Myo1d Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHybr_r2a>Fab-srt-his

    Plasmid

    #124810

    Purpose

    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    rat IgG2a Hinge-G4S-Sortag-Histag
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial

    Mutation

    Promoter

    pUC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FH95pUP95S73AGW (F2)

    Plasmid

    #74039

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusion

    Depositor

    Bernardo Sabatini, Weifeng Xu, Robert Malenka

    Article

    Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    S73A

    Promoter

    H1

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

13. FH95pUP95Q15AD45_64GW (D8)

    Plasmid

    #74031

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-Q15A _45_64-EGFP fusion

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Q15A, D45_64

    Promoter

    H1

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

14. FH95pUP95L460pGW (E2)

    Plasmid

    #74032

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-L460P-EGFP fusion

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    L460P

    Promoter

    H1

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

15. FUP95Q15AL460PGW (E3)

    Plasmid

    #74033

    Purpose

    lentiviral expression of Psd95-Q15A L460P-EGFP fusion

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Q15A, L460P

    Promoter

    hUbC

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

16. FH95pUP95DSKIrGW (D2)

    Plasmid

    #74025

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95dSK and EGFP

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    delta SK

    Promoter

    H1

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

17. FUP95DPESTGW (C7)

    Plasmid

    #74023

    Purpose

    lentiviral expression of Psd95-DPEST-EGFP fusion

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    DPEST; amino acids 10-25 deleted

    Promoter

    hUbC

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

18. Rescue myc-PCDHGC5 mRNA

    Plasmid

    #122229

    Purpose

    Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.

    Depositor

    Angel de Blas

    Article

    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

    Insert

    PCDHGC5 (Pcdhgc5 Rat)
    

    Use

    Tags

    9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

    Expression

    Mammalian

    Mutation

    Silent mutations are in five consecutive codons …

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

    Plasmid

    #122450

    Purpose

    Expression of inositol 1,4,5-trisphosphate 3-kinase A

    Depositor

    Christophe Erneux

    Article

    Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

    Insert

    inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
    

    Use

    Tags

    beta-galactosidase

    Expression

    Bacterial

    Mutation

    coding sequence from V66 till stop codon

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Myc-gamma3/gamma2SIL

    Plasmid

    #121433

    Purpose

    GABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3/GABRG2 SIL (Gabrg2, Gabrg3 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge