2,294 results

1. pAAV-TetO(3G)-GCaMP6f-WPRE

   Plasmid

   #186205

   Purpose

   Express GCaMP6f under TetO(3G)

   Depositor

   Naoshige Uchida

   Article

   Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis (N terminal on insert), T7 epitope, and Xpr…

   Promoter

   TetO(3G)

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-mCherry-SynaptoZip

   Plasmid

   #177318

   Purpose

   AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

   Depositor

   Antonio Malgaroli

   Article

   Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

   Insert

   mCherry-SynaptoZip (Vamp2 Rat)
   

   Use

   AAV

   Tags

   Myc and mCherry

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUGW-shRIIα

   Plasmid

   #183454

   Purpose

   sgRNA targeting rat PKA-RIIα subunits

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA targeting rat PKA-RIIα (Prkar2a Rat)
   

   Use

   Lentiviral

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUGW-scrambled

   Plasmid

   #183455

   Purpose

   This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   scrambled sgRNA
   

   Use

   Lentiviral

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

   Plasmid

   #183457

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

   Plasmid

   #183458

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORANGE_Tubb3 GFP(Y39TAG) KI

   Plasmid

   #182678

   Purpose

   Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and chicken beta-actin promoter

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. iLID-mRuby3-iTrkA

   Plasmid

   #136510

   Purpose

   Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)

   Depositor

   Bianxiao Cui

   Article

   Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

   Insert

   iLID mRuby3 iTrkA (Ntrk1 Avena sativa, Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLZRS-mVenus-TrkC

   Plasmid

   #182948

   Purpose

   To express the rat TrkC fused in the N-terminus with mVenus

   Depositor

   Pantelis Tsoulfas

   Insert

   mVenus-TrkC (Ntrk3 Synthetic, Rat)
   

   Use

   Retroviral and Synthetic Biology

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   MMLV

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLZRS-TrkC-mCherry

    Plasmid

    #182949

    Purpose

    To express the rat TrkC fused in the C-terminus with mCherry

    Depositor

    Pantelis Tsoulfas

    Insert

    TRk-C-mCherry (Ntrk3 Synthetic, Rat)
    

    Use

    Retroviral and Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    MMLV

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBBK54 pHomL-RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

    Plasmid

    #179038

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-2xPH(PLCd), a marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE Tubb3-2xGFP KI

    Plasmid

    #183443

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 2xGFP donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOC4 Kcnn2-smFP-myc KI

    Plasmid

    #183438

    Purpose

    FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP myc donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOC2 GFP-Mpp2 KI

    Plasmid

    #183434

    Purpose

    CreOFF knock-in for GFP-MPP2 (amino acid position: A4)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOC5 smFP-HA-Cacna1e KI

    Plasmid

    #183441

    Purpose

    FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP HA donor
    

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOC2 Gria1-Halo KI

    Plasmid

    #183433

    Purpose

    CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo donor
    

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORANGE Tubb3-3xGFP KI

    Plasmid

    #183444

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 3xGFP donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOC2 Tubb3 no donor

    Plasmid

    #183431

    Purpose

    CreOFF gRNA expression for beta3-tubulin (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA
    

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOC2 GFP-Actb KI

    Plasmid

    #183429

    Purpose

    CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOC4 Grin1-smFP-V5 KI

    Plasmid

    #183439

    Purpose

    FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP V5 donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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