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Plasmid#216161PurposeContains a Golden-Gate cloning cassette to express up to four gRNA and EGFPDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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HPV-31neo
Plasmid#153283PurposeNeomycin selectable HPV31 genomeDepositorInsertHPV-31 genome with Neo cassette
UseUnspecifiedTagsExpressionMutationHindIII and CsiI site mutated from original Neo c…PromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Expression scaffold for dCas9+sgRNA
Plasmid#171799PurposePICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNADepositorInsertPICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-HHARI(1-185)
Plasmid#17341DepositorInsertHHARI (ARIH1 Human)
UseTagsFlagExpressionMammalianMutationAmino acids 1-185PromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBA52_Ubiquitin
Plasmid#110043PurposeProtein expression and purification of UBA52_UbiquitinDepositorInsertUBA52_Ubiquitin (UBA52 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC-hTDG-K330A-PreE2
Plasmid#52273PurposeBacterial expression of human TDG-K330A C-terminally fused to SUMO-E2 and GSTDepositorInserthTDG (TDG Human)
UseTagsUbc9-GSTExpressionBacterialMutationK330A, SUMO acceptor site mutationPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-hTDG-IntE2
Plasmid#52270PurposeBacterial expression of human TDG C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInserthTDG (TDG Human)
UseTagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
empty_pK7fWG2_IVD_FP611
Plasmid#228371PurposeEmpty dual expression vector for determining subcellular localization for protein of interestDepositorTypeEmpty backboneUseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
EKAREN5-gl
Plasmid#225958PurposeThe YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.DepositorInsertEKAREN5-gl
UseLentiviralTagsnls localization motifExpressionMammalianMutationPromoterEF-1-alpha promoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmCherryC1-Pacer
Plasmid#216698PurposeMammalian expression of PacerDepositorInsertPacer (RUBCNL Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-GFP
Plasmid#209399PurposeTransiently expressing and visualizing the subcellular localization of TurboID in plantaDepositorInsert3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5’UTR-ORF1
Plasmid#190566PurposeHolding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).DepositorInsertContiguous regions of the human L1 (L1.3) 5’UTR and ORF1
UseFor dna propagation onlyTagsExpressionBacterialMutationPromoterN/AAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Negative feedback (NF) plasmid
Plasmid#169747PurposeStandalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.DepositorInserttetR-P2A-eGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-ysg(NT)
Plasmid#138263PurposeSubcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.DepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
YCe1885 HC_Kan_Kozak-ATG-MLS_p6
Plasmid#100676Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertKozak-ATG-MLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
YCe2735 HC_Kan_Kozak-ATG-IgkL_p6
Plasmid#100697Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertKozak_ATG_IgkL
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2B08
Plasmid#70910PurposeGateway entry cloneDepositorInsertUba2 (Uba2 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC-hXRCC1-K176R-PreE2
Plasmid#52279PurposeBacterial expression of human XRCC1-K176R C-terminally fused to SUMO-E2 and GSTDepositorInsertXRCC1 (XRCC1 Human)
UseTagsUbc9-GSTExpressionBacterialMutationK176R, SUMO acceptor site mutantPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-hXRCC1-IntE2
Plasmid#52276PurposeBacterial expression of human XRCC1 C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInsertXRCC1 (XRCC1 Human)
UseTagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP
Plasmid#196988PurposeDerived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vectorDepositorInsertNestin-dCas9-KRAB-T2a-GFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only