We narrowed to 59,329 results for: cloning

1. KTK_324

   Plasmid

   #180566

   Purpose

   Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
   

   Expression

   Bacterial

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBtet-GP

   Plasmid

   #60495

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   TCE & RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEZT-BM

   Plasmid

   #74099

   Purpose

   Multi-purpose rapid titer bacmam expression vector

   Depositor

   Ryan Hibbs

   Article

   Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Bacmam, baculovirus

   Tags

   EGFP following p10 promoter

   Expression

   Mammalian

   Promoter

   CMV, p10

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PresentER-Cassette mCherry

   Plasmid

   #102943

   Purpose

   PresentER with removable cassette for cloning of MHC-I antigen (mCherry version)

   Depositor

   David Scheinberg

   Article

   Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest-676 (attR4-attR3)

   Plasmid

   #161888

   Purpose

   Multisite Gateway (attR4-attR3) vector for lentivirus production

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

   Plasmid

   #71485

   Purpose

   Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Type

   Empty backbone

   Promoter

   U6

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hs.HRAS G12V

   Plasmid

   #83184

   Purpose

   Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12V mutation

   Depositor

   Dominic Esposito

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12V

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV PIG (Puro IRES GFP empty vector)

   Plasmid

   #21654

   Purpose

   Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC28-LIC-sfGFP-His

   Plasmid

   #165479

   Purpose

   pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

   Depositor

   Renato Bruni

   Article

   Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

   Type

   Empty backbone

   Tags

   TEV-GFP-6xHis

   Expression

   Bacterial

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETMCSI-T4P_Y320A

    Plasmid

    #122522

    Purpose

    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

    Depositor

    Gottfried Otting

    Article

    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

    Insert

    gp43 - DNA polymerase (43 Escherichia virus T4)
    

    Expression

    Bacterial

    Mutation

    tyrosine 320 changed to alanine

    Promoter

    T7 promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PresentER-Cassette GFP

    Plasmid

    #102942

    Purpose

    PresentER with removable cassette for cloning of MHC-I antigen (GFP version)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGGF007

    Plasmid

    #48847

    Purpose

    Provides a kanamycin resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pNOS::KanamycinR::tNOS
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pREXC3GH

    Plasmid

    #47077

    Purpose

    FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Lactococcus lactis expression

    Tags

    3C protease site, GFP, and His

    Promoter

    NIS (nisin)

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJW1310

    Plasmid

    #61253

    Purpose

    Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    U6 promoter
    

    Use

    CRISPR; Cloning template

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSC27

    Plasmid

    #117478

    Purpose

    mobilizable, SRB-stable, kanamycin-resistant cloning vector

    Depositor

    Judy Wall

    Article

    Rousset et al Plasmid. 1998;39(2):114-22. doi: 10.1006

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. DDB1

    Plasmid

    #25246

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DDB1_1 (DDB1 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pJW1311

    Plasmid

    #61254

    Purpose

    Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E) targeting Y61A9L9.1
    

    Use

    CRISPR; Cloning template

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME Integrin beta 1b

    Plasmid

    #105976

    Purpose

    entry clone for gateway cloning, Full length Integrin beta 1b without stop

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Integrinbeta1b
    

    Expression

    Bacterial

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBXCA3H

    Plasmid

    #47072

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), Avi-tag, and…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBXNH3CA

    Plasmid

    #47069

    Purpose

    FX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), AVI-tag, and…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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