We narrowed to 5,433 results for: dre

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pNHT7::RPL15::sNluc2_STOP

Plasmid

#186767

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Mutation

Premature stop codon at residue 16

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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puc19_N_tag

Plasmid

#186650

Purpose

Empty donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

N-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-R89L-nTEVp

Plasmid

#179670

Purpose

Mutant Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

nTEVP

Expression

Mammalian

Mutation

K65E, R89L

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-R89L-cTEVp

Plasmid

#179671

Purpose

Mutant Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

cTEVP

Expression

Mammalian

Mutation

K65E, R89L

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-HRAS-G12V

Plasmid

#179652

Purpose

HRAS active mutant (G12V mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

HRAS (HRAS Human)

Expression

Mammalian

Mutation

G12V

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Miro1 (184-618)

Plasmid

#127626

Purpose

Human Miro1 lacking the first GTPase domain

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Miro1 (RHOT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

184-618 only

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2001

Plasmid

#105871

Purpose

High copy HRPT backbone plasmid for insertion of MiniP promoters

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Type

Empty backbone

Available Since

Feb. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pOCC176

Plasmid

#118891

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEV

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6-MBP, cleavable with 3C protease; SNAP, cleav…

Expression

Insect

Promoter

polH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-LAP-BUB1

Plasmid

#115903

Purpose

Used for expression of siRNA-resistant BUB1-WT from the FRT site of FlpIn cells upon addition of doxycyclin.

Depositor

Geert Kops

Article

Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

Insert

BUB1 (BUB1 Human)

Tags

EYFP

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-BirA-UBE3B-Puro

Plasmid

#107351

Purpose

Expresses UBE3B fused to BirA in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

BirA-UBE3B (UBE3B Human)

Use

Lentiviral

Tags

Biotin ligase

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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KHBD00676

Plasmid

#39724

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG42277 (rn Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00574

Plasmid

#39660

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6534 (slou Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00697

Plasmid

#39734

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17390 (Oaz Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00626

Plasmid

#39698

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10197 (kn Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00623

Plasmid

#39695

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17594 (scro Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00524

Plasmid

#39624

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG13493 (comr Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00705

Plasmid

#39740

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5586 (Tusp Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00645

Plasmid

#39707

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17100 (cwo Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00646

Plasmid

#39708

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1705 (Met Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00607

Plasmid

#39685

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG32788 (Crg-1 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only