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We narrowed to 9,067 results for: sgRNA

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Showing: 2021 - 2040 of 9067 results

  7. pGG422_UV5

    Plasmid
    #165607
    Purpose
    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1332K and R1333G mutations in SpCas9
    Promoter
    lacUV5 driving Cas9 KG and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165607-plasmid-map-sequence-id-330962

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  8. lentiCRISPR - EGFP sgRNA 4

    Plasmid
    #51763
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    51763-plasmid-map-sequence-id-79382

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  14. pX330-CANX

    Plasmid
    #227279
    Purpose
    Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting C-terminus of CANX (CANX Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227279-plasmid-map-sequence-id-451277

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  18. Direct-seq_lentiGuide-Puro-Loop2-8A8G

    Plasmid
    #157983
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Publication
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Loop2-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157983-plasmid-map-sequence-id-332422

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  19. pMH183

    Plasmid
    #122856
    Purpose
    MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences
    Depositor
    Markus Schmid
    Publication
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    Use
    Golden gate compatible cloning vector
    Available since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122856-plasmid-map-sequence-id-247655

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Showing: 2021 - 2040 of 9067 results