We narrowed to 41,926 results for: Tro;
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RCAN1-aa1-150
Plasmid#65412PurposeRCAN1 aa1-150 expression with CMV promoter. Flag tagged.DepositorInsertRCAN1-aa1-150 (Rcan1 Mouse)
TagsFlagExpressionMammalianMutationaa1-150 present, deleted 151 to endAvailable SinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-Dm-miR-279
Plasmid#22707DepositorInsertmicro RNA 279 (mir-279 Fly, primary transcript)
UseDrosophila expressionAvailable SinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRVH1-hygro shStx3
Plasmid#40071DepositorInsertStx3 shRNA-1
UseRetroviralPromoterH1Available SinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Diap3 in situ probe
Plasmid#45602DepositorInsertDiap3 in situ probe (Diaph3 Mouse)
UseIn situMutationfragment contains bp# 1589-2321 of BC028920Available SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Crim1 in situ probe
Plasmid#45600DepositorInsertCrim1 in situ probe (Crim1 Mouse)
UseIn situMutationinsert contains bp#4062-4502 of NM_015800.3Available SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2+t-gfp
Plasmid#48132PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MBD4_1
Plasmid#36374DepositorAvailable SinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
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LentiGuide Cyclone-Puro P2A EGFP
Plasmid#247497PurposeAcyclovir-regulated puromycin n-acetyltransferase-P2A-EGFP fusion proteinDepositorInsertpuromycin n-acetyltransferase with Cyclone insertion
UseLentiviral and Synthetic BiologyTagsPuro(Cyclone)-P2A-EGFPExpressionMammalianMutationCyclone was inserted after Glutamine135 of puromy…PromoterEF-1aAvailable SinceDec. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CR-QUAS:NTR-mKate2
Plasmid#241914PurposeZebrafish transgenesis plasmid encoding nitroreductase-mKate2, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis markerDepositorInsertQUAS enhancer driving nitroreductase-mKate2
UseZebrafish tol2 transgenesisTagsnitroreductase fused with mKate2PromoterQUASAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pREC1010-opEas1
Plasmid#240701PurposeRSF1010 origin of replication plasmid containing Eas1 recombitron with extended a1 a2 regions in the ncRNA targeting lacZ locus in Klebsiella pneumoniae ATCC 10031 expressed by Pm promoterDepositorInsertEas1 RT, Eas1 ncRNA, CspRecT and EcSSB
ExpressionBacterialMutationExtended a1 a2 regionsAvailable SinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Split PS Intein gal4-vp16
Plasmid#242030PurposeBlue-light-controlled release of gal4-vp16 transactivator from the cytoplasm to the nucleus. Split version.DepositorInsertSplit PS Intein gal4-vp16
ExpressionMammalianMutationpNL3.2.NF-KB-RE[NlucP/NF-KB-RE/Hygro]PromoterCMVAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBIRC5-Split PS Intein-N tTA
Plasmid#242033PurposeN-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the BIRC5 promoter.DepositorInsertSplit PS Intein-N tTA
TagsmTagBFP2ExpressionMammalianMutationThe CMV promoter was replaced with the BIRC5 prom…PromoterhTERTAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
phTERT-Split PS Intein-N tTA
Plasmid#242034PurposeN-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the hTERT promoter.DepositorInsertSplit PS Intein-N tTA
TagsmTagBFP2ExpressionMammalianMutationThe CMV promoter was replaced with the hTERT prom…PromoterhTERTAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRECBRR1-Kva1
Plasmid#240703Purposeinverted BRR1 origin of replication plasmid containing Kva1 recombitron with a donor in the ncRNA to target phzM locus in Pseudomonas aeruginosa PAO1 expressed by Pm promoterDepositorInsertKva1 RT,Kva1 ncRNA, PaRecT and PaSSB
ExpressionBacterialMutationInverted BRR1 origin of replicationAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pREC1010C-Eco1
Plasmid#240690PurposeRSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by J23115 constitutive promoterDepositorInsertEco1 RT, Eco1 ncRNA, CspRecT and EcSSB
ExpressionBacterialMutationWTAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8-cyp-33e1-flag-tev-halo-his
Plasmid#227151PurposeFor T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.DepositorInsertcyp-33e1 (cyp-33E1 Nematode)
TagsFlag, HaloTag, and His6ExpressionMammalianPromoterCMV and SP6Available SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8-CYP2A6-flag-tev-halo-his
Plasmid#227154PurposeFor T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.DepositorAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCR197
Plasmid#240643PurposeExpression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymeraseDepositorInsertsAIPGENE865 promoter
SP6 promoter
AmNeonGreen
mTurquoise2
mScarlet-I
UseGene expression and genomic integration in fishTagsNES (from Exaiptasia diaphana MEK2), NLS (from SV…MutationCodon-optimized for Exaiptasia diaphana, amino ac…Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pREC1010-Abe1
Plasmid#240683PurposeRSF1010 origin of replication plasmid containing Abe1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoterDepositorInsertAbe1 RT, Abe1 ncRNA, CspRecT and EcSSB
ExpressionBacterialMutationWTAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pALN132
Plasmid#239799PurposeExpress mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domainDepositorInsertMmCENPT--Rn-Histone Fold Domain
UseRetroviralTagsmEGFPExpressionMammalianPromoterMoloney Murine Leukemia Virus (MMLV) LTR promoterAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-Trim21-OLLAS
Plasmid#237438PurposeExpresses mouse Trim21 ubiquitin ligase tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)DepositorAvailable SinceJune 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENP-W
Plasmid#237433PurposeExpresses Mus musculus CENP-W; untagged; made for in vitro transcriptionDepositorAvailable SinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENPT-GFP
Plasmid#237426PurposeExpresses Mus musculus CENP-T tagged with GFP at C-term; made for in vitro transcription (T7 promoter)DepositorAvailable SinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCARGO-RMCE-Airn
Plasmid#233266PurposeCARGO plasmid containing ~90kb dox-inducible Airn transgeneDepositorInsertAirn (Airn Mouse)
ExpressionMammalianAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT478
Plasmid#228286PurposeConstitutive mutant rPhlTA expression with strong promoterDepositorInsertrphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40
Plasmid#233368PurposehSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transductionDepositorInsertNES-Kinprola_on-mEGFP
UseAAVTagsNES and mEGFPPromoterhSyn1Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40
Plasmid#233367PurposehSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transductionDepositorInsertNES-Kinprola_PKA_T/A-mEGFP
UseAAVTagsNES and mEGFPPromoterhSyn1Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_off-mEGFP
Plasmid#233356PurposeCMV driven expression of the inactive kinase activity recorder negative control Kinprola_off in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_off-mEGFP
TagsNES and mEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKA_T/A-mEGFP
Plasmid#233354PurposeCMV driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKA_T/A-mEGFP
TagsNES and mEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_JNK_T/A-mEGFP
Plasmid#233360PurposeCMV driven expression of the inactive JNK activity recorder negative control with T/A mutation Kinprola_JNK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_JNK_T/A-mEGFP
TagsNES and mEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKC_T/A-mEGFP
Plasmid#233358PurposeCMV driven expression of the inactive PKC activity recorder negative control with T/A mutation Kinprola_PKC_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC_T/A-mEGFP
TagsNES and mEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP
Plasmid#233355PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_on-mEGFP
TagsNES and mEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP
Plasmid#233362PurposeCMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK_T/A-mEGFP
TagsNES and mEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP
Plasmid#233370PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_on-mEGFP
UseLentiviralTagsNES and mEGFPPromoterCMVAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Nat-ADE2
Plasmid#232106PurposeExpresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterZ3Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Hyg-ADE2
Plasmid#232104PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Hyg-ADE2
Plasmid#232107PurposeExpresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterZ3Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS-miRFP670-TRF1
Plasmid#227378PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, WT (TERF1 Human)
UseLentiviralAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
iLID-miRFP670-TRF1
Plasmid#227385PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric iLID (TERF1 Human)
UseLentiviralAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-NS3/4A S139A
Plasmid#186885PurposeDoxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transductionDepositorInsertHCV NS3/4A protease with S139A mutation
UseRetroviralMutationS139APromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-WHG cGAS-HA
Plasmid#186867PurposeDoxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transductionDepositorInsertWhite-handed gibbon cGAS with C-terminal HA tag
UseRetroviralTagsHAPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2
Plasmid#221082PurposePlasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.DepositorInsertACC-msfGFP[r5M]_ACC-mEBFP2
UseSynthetic BiologyPromoterCMV, T7Available SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-WzzE
Plasmid#196661PurposeExpression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
TagsTEV-cleavable His6ExpressionBacterialPromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGST-WzzE
Plasmid#196662PurposeExpression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
TagsTEV-cleavable GSTExpressionBacterialMutationValine insertion at position 2.PromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-mCherry-WzzE
Plasmid#196663PurposeExpression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
TagsHis and mCherryExpressionBacterialMutationValine insertion at position 2 of WzzE.PromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-GST-distrepII
Plasmid#196666PurposeExpression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
TagsGST and TEV-cleavable di-strep tag IIExpressionBacterialPromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-strep-mClover3
Plasmid#196668PurposeExpression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
TagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialPromoteraraBADAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4QE2Crimson
Plasmid#218280PurposeIntroduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoterDepositorInsertpURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only
