We narrowed to 3,856 results for: biorxiv

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pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

V205F

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelCTerm_1-223AA

Plasmid

#221476

Purpose

Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

Deletion of 224-371AA C-terminal domain

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelNTerm_160-371AA

Plasmid

#221475

Purpose

Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

Deletion of 1-159AA N-terminal domain

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelNTerm_160-371AA

Plasmid

#221487

Purpose

NKX2-1 N-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX-EF1a_NKX2-1_V205F

Plasmid

#221485

Purpose

NKX2-1 V205F DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V205F

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX-SFFV_NKX2-1

Plasmid

#221496

Purpose

NKX2-1 short isoform lentiviral overexpression using SFFV promoter

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

SFFV

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNIC-OmpA-NHSTIIT-GG

Plasmid

#215790

Purpose

Cloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-SufI-NHSTIIT-GG

Plasmid

#215791

Purpose

Cloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

Plasmid

#202717

Purpose

Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging

Depositor

Adam Cohen

Publication

Xiang et al bioRxiv 2023

Inserts

Myr-Pink Flamindo
EGFP-bPAC

Use

Lentiviral

Tags

10xHis and myc

Expression

Mammalian

Promoter

CMV

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SV40-iBlue-pA

Plasmid

#200234

Purpose

Backbone for cloning iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pminiT-MED6-mEmerald donor

Plasmid

#197865

Purpose

Donor plasmid used for building MED6-mEmerald knock-in mESCs

Depositor

Zhe J. Liu

Publication

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Use

Mouse Targeting

Tags

mEmerald

Promoter

NA

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pminiT-mEmerald-MED1 donor

Plasmid

#197867

Purpose

Donor plasmid used for building mEmerald-MED1 knock-in mESCs

Depositor

Zhe J. Liu

Publication

Dong et al bioRxiv 2022.10.26.513917

Insert

CRSP210 (Med1 Mouse)

Use

Mouse Targeting

Tags

mEmerald

Promoter

NA

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODALvar

Plasmid

#115640

Purpose

For targeted integration and inducible expression of a human NODAL splice variant using doxycycline

Depositor

Lynne Postovit

Publication

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-matDYK

Plasmid

#115258

Purpose

For mammalian expression of the human NODAL open reading frame with an internal DYK tag

Depositor

Lynne Postovit

Publication

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-matNODALvar-HIS

Plasmid

#115265

Purpose

For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

Depositor

Lynne Postovit

Publication

Findlay et al (2018) bioRxiv 276170

Insert

NODAL variant mature peptide (NODAL Human)

Tags

6His

Expression

Bacterial

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-T7TS

Plasmid

#115251

Purpose

For in vitro transcription of a human NODAL splice variant open reading frame RNA

Depositor

Lynne Postovit

Publication

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Bacterial

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTbp-Firefly

Plasmid

#177835

Purpose

Luciferase reporter for mouse Tbp

Depositor

Alexander Ruthenburg

Publication

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Promoter

Tbp

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-Chantico

Plasmid

#170659

Purpose

pUC19 vector containing an insertion of the Chantico promoter

Depositor

Alexander Ruthenburg

Publication

Hall et al bioRxiv 2021.12.23.474040

Insert

Chantico

Use

Cloning

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pChantico-Firefly

Plasmid

#163901

Purpose

Luciferase reporter for Chantico lncRNA

Depositor

Alexander Ruthenburg

Publication

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Promoter

Chantico

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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