We narrowed to 3,663 results for: golden gate

1. L1p4_barley_sgRNA_scaffold

   Plasmid

   #231031

   Purpose

   Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 4

   Depositor

   Wilma van Esse

   Article

   Winkelmolen et al bioRxiv 2024.08.22.609225

   Insert

   Scaffold for Cas9 mediated CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TaU6

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. L1p3_barley_sgRNA_scaffold

   Plasmid

   #231030

   Purpose

   Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3

   Depositor

   Wilma van Esse

   Article

   Winkelmolen et al bioRxiv 2024.08.22.609225

   Insert

   Scaffold for Cas9 mediated CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TaU6

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Zdk3-VPR

   Plasmid

   #231572

   Purpose

   Expresses components of the LOOMINA optogenetic transcriptional control system.

   Depositor

   Dominik Niopek

   Article

   Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

   Insert

   Zdk3-VPR, Ef1a driven
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEVA26_sgRNA-A0_M13ps

   Plasmid

   #231413

   Purpose

   M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Insert

   sgRNA-A0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PAF24

   Plasmid

   #223172

   Purpose

   Expresses VC83_00191-yeGFP under control of CTR1 promoter and terminator

   Depositor

   Ryan Peterson

   Article

   Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

   Insert

   Copper Transporter
   

   Use

   Tags

   StreptagII and yeGFP

   Expression

   Yeast

   Mutation

   Promoter

   saccharomyces cerevisiae CTR1

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHW622

   Plasmid

   #229868

   Purpose

   Entry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)

   Depositor

   Han Wang

   Article

   Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

   Insert

   reverse FRT3 (rFRT3)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MbCas12a_K487_GPG_GR2

   Plasmid

   #231577

   Purpose

   Encodes a cortisol inducible variant of MbCas12a

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   MbCas12a with a GR2 insertion behind K487
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. cpLOV2-Gal4

   Plasmid

   #231569

   Purpose

   Expresses components of the LOOMINA optogenetic transcriptional control system.

   Depositor

   Dominik Niopek

   Article

   Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

   Insert

   cpLOV2-Gal4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV_Zdk3-VPR

   Plasmid

   #231573

   Purpose

   Expresses components of the LOOMINA optogenetic transcriptional control system.

   Depositor

   Dominik Niopek

   Article

   Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

   Insert

   Zdk3-VPR, CMV driven
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMK83

    Plasmid

    #226793

    Purpose

    Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Insert

    slr0214 (slr0214 Synechocystis sp. PCC 6803)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tetracycline resistance cassette promoter (consti…

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGGA016

    Plasmid

    #228231

    Purpose

    Level 0 for 5xOp (GreenGate A module)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    5xOp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCHW111111

    Plasmid

    #222456

    Purpose

    Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    CBS4-miniDFR (Solanum lycopersicum)

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCHW111112

    Plasmid

    #222457

    Purpose

    Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    CBS4-miniDFR

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCHW00015

    Plasmid

    #222446

    Purpose

    Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    VP64-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCHW91069

    Plasmid

    #222462

    Purpose

    Level 1 acceptor, Position 3 containing CBS4-miniDFR::mCherry2-HyP5SM-LacZ::nosT.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    CBS4-miniDFR (Solanum lycopersicum)

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCHW101008

    Plasmid

    #222453

    Purpose

    Level 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    CBS4-miniDFR::OsL5::NOS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCHW00014

    Plasmid

    #222445

    Purpose

    Level 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    VP16-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHW110034

    Plasmid

    #222443

    Purpose

    Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    LoxP-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid

    #227645

    Purpose

    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

    Depositor

    Richard Murray

    Article

    Kapasiawala et al bioRxiv 2023.03.22.533877

    Insert

    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTBL2142 MTK3-sfGFP

    Plasmid

    #226661

    Purpose

    MTK3 part containing the coding sequence of sfGFP

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge