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We narrowed to 40,548 results for: tro

Showing: 4121 - 4160 of 40548 results

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pcDNA4/TO-2xStrep-ORF68 K435A

Plasmid

#162636

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K435A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid K435 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 R443A

Plasmid

#162637

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 R443A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid R443 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 K450A/R451A

Plasmid

#162638

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K450A/R451A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids K450 and R451 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 R14A

Plasmid

#162640

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 R14A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid R14 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 K310A

Plasmid

#162641

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K310A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid K310 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 R14A/K310A

Plasmid

#162642

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 R14A/K310A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids R14 and K310 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 K395A/K396A

Plasmid

#162643

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K395A/K396A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids K395 and K396 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 C128A

Plasmid

#162644

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C128A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C128 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 C502A

Plasmid

#162645

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C502A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C502 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 C308A/C309A

Plasmid

#162646

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C308A/C309A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids C308 and C309 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 C544A

Plasmid

#162647

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C544A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C544 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 H581A

Plasmid

#162648

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 H581A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid H581 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 C155A

Plasmid

#162649

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C155A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C155 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 C52A

Plasmid

#162630

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C52A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C52 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 C373A

Plasmid

#162631

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C373A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C373 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 C191A/C192A

Plasmid

#162632

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C191A/C192A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids C191 and C192 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pKM54

Plasmid

#154157

Purpose

Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB site

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

8 copies of the Aedes aegypti Rel2 NF-κB site

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pKM44

Plasmid

#154153

Purpose

Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Drosophila melanogaster minimal Hsp70 promoter

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBz106_CsoS2_MR

Plasmid

#140843

Purpose

His-tagged CsoS2 Middle Region (MR), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 Middle Region (MR)

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

only amino acids 257-603

Promoter

T7

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLz75_CB_operon_CsoS2_N3-N4

Plasmid

#140857

Purpose

Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removed

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids 1-109 of CsoS2

Promoter

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLz77_CB_operon_CsoS2_N3

Plasmid

#140859

Purpose

Same as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removed

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids 1-108 and 220-235 of CsoS2

Promoter

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz78_CB_operon_CsoS2_delNTD

Plasmid

#140860

Purpose

Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids 1-235 of CsoS2

Promoter

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz76_CB_operon_CsoS2_N4

Plasmid

#140858

Purpose

Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids 1-189 of CsoS2

Promoter

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBz109_CsoS2_NTD

Plasmid

#140842

Purpose

His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 N-terminal domain (NTD)

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 262-869

Promoter

T7

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz33_CsoS2_NTD_GFP

Plasmid

#140861

Purpose

His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD fused to GFP

Use

Tags

Hexahistidine tag and Superfolder GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz47_polyPro_CsoS2_Npeptide

Plasmid

#140845

Purpose

Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 N-peptide

Use

Tags

Hexahistidine tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBz110_CsoS2_CTD

Plasmid

#140844

Purpose

His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 C-terminal domain (CTD)

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 1-594

Promoter

T7

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 539

Plasmid

#138078

Purpose

Control vector with no degradation tag to compare against various degradation tag variants

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry::sbfI- pSEVA-331Bb

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCk4

Plasmid

#136698

Purpose

Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB4C5_J23101-vdh

Plasmid

#128130

Purpose

Expressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

vdh

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_AtCRY2-PHR_X

Plasmid

#131180

Purpose

Expresses AtCRY2-PHR (Arabidopsis thaliana) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtCRY2-PHR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0652

Plasmid

#129013

Purpose

Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_X_EcSSPB-micro

Plasmid

#131218

Purpose

Expresses EcSSPB-nano (Escherichia coli) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-micro

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_X_EcSSPB-nano

Plasmid

#131219

Purpose

Expresses EcSSPB-micro (Escherichia coli) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_EcSSPB-micro_X

Plasmid

#131213

Purpose

Expresses EcSSPB-micro (Escherichia coli) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-micro

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_X_AtCIB

Plasmid

#131194

Purpose

Expresses AtCIB (Arabidopsis thaliana) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtCIB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_X_AtCRY2-PHR

Plasmid

#131191

Purpose

Expresses AtCRY2-PHR (Arabidopsis thaliana) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtCRY2-PHR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_X_RsLP-LOV

Plasmid

#131188

Purpose

Expresses RsLP-LOV (Rhodobacter sphaeroides) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

RsLP-LOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_X_NcVVD-LOV

Plasmid

#131187

Purpose

Expresses NcVVD-LOV (Neurosporra crassa) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

NcVVD-LOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_X_CrPH-LOV

Plasmid

#131186

Purpose

Expresses CrPh-LOV domain (Chlamydomonas reinhardtii) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

CrPH-LOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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