We narrowed to 2,128 results for: Pam

1. pCAG-mCherry-FKBP-GAP(ARHGAP29)

   Plasmid

   #222632

   Purpose

   Rho inactivation using rapamycin

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
   

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET32a-HD39Q

   Plasmid

   #11514

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 39 glutamines (HTT Human)
   

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 39 glutamines, vector m…

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5-eYFP-FKBP-MAD1-AA

   Plasmid

   #114038

   Purpose

   Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

   Depositor

   Geert Kops

   Article

   Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

   Insert

   Human MAD1 (MAD1L1 Human)
   

   Tags

   eYFP-FKBP12

   Expression

   Mammalian

   Mutation

   MAD2 binding deficient MAD1 K541A/L543A

   Promoter

   CMV

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LLPS1-iLID::EGFP::FTH1 (pBS1042)

   Plasmid

   #185289

   Purpose

   For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   iLID::eGFP::FTH1
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. DRD5-DuET

   Plasmid

   #213231

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   DRD5 (DRD5 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ApoE3_mCh-SspB (pBS1144)

   Plasmid

   #185326

   Purpose

   For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE3
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FKBP-DCRD-YFP

   Plasmid

   #87450

   Purpose

   rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

   Depositor

   Xiaodong Liu

   Article

   Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

   Insert

   DCRD (distal C-terminal regulatory domain) (CACNA1F Human)
   

   Tags

   6xHis-T7, FKBP, and YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ApoE2_mCh-SspB (pBS1142)

   Plasmid

   #185324

   Purpose

   For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE2
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CFP-PCRD-FRB

   Plasmid

   #87449

   Purpose

   rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

   Depositor

   Xiaodong Liu

   Article

   Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

   Insert

   PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
   

   Tags

   6xHis-T7, CFP, and FRB

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid

    #222876

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FRB chain: V84R in GPA

    Promoter

    MSCV LTR

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LBR(R583Q)-FKBP-GFP

    Plasmid

    #222423

    Purpose

    To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    LBR(R583Q) (LBR Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    R583Q

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LBR(N547D)-FKBP-GFP

    Plasmid

    #222422

    Purpose

    To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    LBR(N547D) (LBR Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    N547D

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DRD3-DuET

    Plasmid

    #213229

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    DRD3 (DRD3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

    Plasmid

    #185299

    Purpose

    Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_shuffle_3
    

    Expression

    Mammalian

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DHN_VrDHN1a_mCh-SspB (pBS1074)

    Plasmid

    #185298

    Purpose

    Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k3_26
    

    Expression

    Mammalian

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ApoE3_D125I_47-300_mCh-SspB (pBS1071)

    Plasmid

    #185294

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I_47-300
    

    Expression

    Mammalian

    Mutation

    D125I

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ApoE3_D125I_mCh-SspB (pBS1145)

    Plasmid

    #185327

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I
    

    Expression

    Mammalian

    Mutation

    D125I

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ApoE4_mCh-SspB (pBS1143)

    Plasmid

    #185325

    Purpose

    For the mammalian expression of the human protein ApoE4 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE4
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)

    Plasmid

    #185309

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I_47-300
    

    Expression

    Mammalian

    Mutation

    D125I

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pvLEA22mer_mutant_3_mCh-SspB (pBS1080)

    Plasmid

    #185303

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_mutant_3
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge