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pFR093

Plasmid

#134149

Purpose

Expression of CENP-T

Depositor

Iain Cheeseman

Article

Nishino et al EMBO J. 2013 Feb 6;32(3):424-36. doi: 10

Insert

CENP-T (CENPT Human)

Tags

His

Expression

Bacterial

Mutation

aa 1-375 with T85D mutation

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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JK305: pMVP/Blast-DEST

Plasmid

#121855

Purpose

pMVP destination vector, empty expression vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JL104: pMVP/Neo-DEST

Plasmid

#121857

Purpose

pMVP destination vector, empty expression vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS

Plasmid

#121827

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-Cas9 (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY001: pMVP (L3-L2) BioID2 + polyA

Plasmid

#121795

Purpose

pMVP L3-L2 entry plasmid, contains BioID2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

BioID2 + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY202: pMVP (L3-L2) APEX2 + polyA

Plasmid

#121796

Purpose

pMVP L3-L2 entry plasmid, contains APEX2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

APEX2 + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JD602: pMVP (L3-L2) APEX2 + WPRE

Plasmid

#121798

Purpose

pMVP L3-L2 entry plasmid, contains APEX2 + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

APEX2 + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC601: pMVP (L3-L2) eYFP + polyA

Plasmid

#121769

Purpose

pMVP L3-L2 entry plasmid, contains eYFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC801: pMVP (L3-L2) eGFP + WPRE

Plasmid

#121771

Purpose

pMVP L3-L2 entry plasmid, contains eGFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC901: pMVP (L3-L2) mCherry + WPRE

Plasmid

#121773

Purpose

pMVP L3-L2 entry plasmid, contains mCherry + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LD001: pMVP (L3-L2) eYFP + WPRE

Plasmid

#121777

Purpose

pMVP L3-L2 entry plasmid, contains eYFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC701: pMVP (L3-L2) eCFP + polyA

Plasmid

#121767

Purpose

pMVP L3-L2 entry plasmid, contains eCFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KG901: pMVP (L3-L2) HA epitope tag + polyA

Plasmid

#121749

Purpose

pMVP L3-L2 entry plasmid, contains HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO102: pMVP (R4-R3) eGFP open

Plasmid

#121731

Purpose

pMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HJ601: pMVP (R4-R3) mCherry open

Plasmid

#121733

Purpose

pMVP R4-R3 entry plasmid, contains mCherry (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO601: pMVP (R4-R3) eCFP open

Plasmid

#121735

Purpose

pMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO401: pMVP (R4-R3) eYFP open

Plasmid

#121737

Purpose

pMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KH305: pMVP (R4-R3) βgal open

Plasmid

#121739

Purpose

pMVP R4-R3 entry plasmid, contains βgal (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

βgal (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LF801: pMVP (L1-R5) cTnT promoter

Plasmid

#121696

Purpose

pMVP L1-R5 entry plasmid, contains cTnT promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

cTnT promoter (Cardiomyocyte specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IH001: pMVP (L5-L4) HA epitope tag

Plasmid

#121699

Purpose

pMVP L5-L4 entry plasmid, contains HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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